Adult healthcare compound protein powder containing tea seed protein and preparation method thereof
A compound protein powder and tea seed protein technology, which is applied in the field of scientific formulation and preparation of tea seed compound protein powder, can solve the problems of rational design and manufacture of essential amino acids, single raw materials, etc., and achieve increased metabolic burden, reasonable composition ratio, and improved The effect of nutritional status on the body
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Embodiment 1
[0019] The defatted tea seed cake is crushed and passed through an 80-mesh sieve, dried at 60°C until the moisture content is less than 5%, and stored for later use. Weigh a certain amount of tea seed powder, mix the tea seed powder with distilled water at a material-to-liquid ratio of 1:20, adjust the pH to 10 with 1mol / L sodium hydroxide solution, stir and extract at 45°C for 80 minutes, and cool to room temperature Afterwards, set the rotation speed to 4500r / min for centrifugation for 15min, and take the supernatant for later use. Transfer the supernatant to another container, and adjust the pH to 4.5 with 1mol / L hydrochloric acid solution for acid precipitation for 20 minutes, then set the speed at 3900r / min for centrifugation for 15 minutes, discard the supernatant, and collect the protein precipitate for later use. Rinse the protein precipitate with deionized water, adjust the pH value of the washed protein to neutral with 0.5mol / L sodium hydroxide solution, dialyze with...
Embodiment 2
[0022] The defatted tea seed cake is crushed and passed through an 80-mesh sieve, dried at 60°C until the moisture content is less than 5%, and stored for later use. Weigh a certain amount of tea seed powder, mix the tea seed powder with distilled water at a material-to-liquid ratio of 1:30, adjust the pH to 9 with 1mol / L sodium hydroxide solution, stir and extract at 50°C for 70 minutes, and cool to room temperature Afterwards, set the rotation speed to 4500r / min for centrifugation for 15min, and take the supernatant for later use. Transfer the supernatant to another container, and adjust the pH to 4.5 with 1mol / L hydrochloric acid solution for acid precipitation for 20 minutes, then set the speed at 3900r / min for centrifugation for 15 minutes, discard the supernatant, and collect the protein precipitate for later use. Rinse the protein precipitate with deionized water, adjust the pH value of the washed protein to neutral with 0.5mol / L sodium hydroxide solution, dialyze with ...
Embodiment 3
[0025] The defatted tea seed cake is crushed and passed through an 80-mesh sieve, dried at 60°C until the moisture content is less than 5%, and stored for later use. Weigh a certain amount of tea seed powder, mix the tea seed powder with distilled water according to the ratio of solid to liquid 1:30, adjust the pH to 10 with 1mol / L sodium hydroxide solution, stir and extract at 50°C for 70min, and cool to room temperature Afterwards, set the rotation speed to 4500r / min for centrifugation for 15min, and take the supernatant for later use. Transfer the supernatant to another container, and adjust the pH to 4.5 with 1mol / L hydrochloric acid solution for acid precipitation for 20 minutes, then set the speed at 3900r / min for centrifugation for 15 minutes, discard the supernatant, and collect the protein precipitate for later use. Rinse the protein precipitate with deionized water, adjust the pH value of the washed protein to neutral with 0.5mol / L sodium hydroxide solution, dialyze ...
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