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Alpine grassland grass endophytic bacteria bacillus amyloliquefaciens strain, microbial agent and preparation method and application thereof

A technology of amylolytic spores and microbial agents, which is applied in the field of microorganisms, can solve the problems of poor safety, poor environmental protection of chemical pesticides, and easy to produce drug resistance, etc., to overcome poor environmental protection, not easy to produce drug resistance, and good safety and environmental protection Effect

Inactive Publication Date: 2017-06-09
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The second object of the present invention is to provide an endophytic bacterium Bacillus amyloliquefaciens microbial inoculum to solve the problems of poor environmental protection, easy to produce drug resistance and poor safety of existing chemical pesticides

Method used

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  • Alpine grassland grass endophytic bacteria bacillus amyloliquefaciens strain, microbial agent and preparation method and application thereof
  • Alpine grassland grass endophytic bacteria bacillus amyloliquefaciens strain, microbial agent and preparation method and application thereof
  • Alpine grassland grass endophytic bacteria bacillus amyloliquefaciens strain, microbial agent and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0027] Embodiment 1: microbial bacterial agent

[0028] Its active ingredient is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) QLY006, the thallus of CGMCCNo.13049, wherein, the content of Bacillus amyloliquefaciens QLY006 is 0.7% by weight percentage, and the culture medium is the balance, and the culture medium is composed of the following substances: Beef extract 2g, sucrose 25g, NaCl 4g, yeast extract 4g, distilled water or deionized water 1000mL, the pH value of described culture medium is 5.5, and the number of viable bacteria of Bacillus amyloliquefaciens QLY006 (CGMCC No.13049) is 1.52×10 10 CFU / mL.

[0029] The preparation method of the above-mentioned microbial bacterial agent is to insert Bacillus amyloliquefaciens QLY006 into the culture medium for fermentation, the insertion amount is 0.7%, the fermentation time is 18h, the fermentation temperature is 24°C, the fermentation speed is 210r / min, and the fermentation solution The oxygen content is 65%.

Embodiment 2

[0030] Embodiment 2: microbial bacterial agent,

[0031] Its active ingredient is the thallus of the above-mentioned Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) QLY006, CGMCC No.13049, which contains 1% by weight of Bacillus amyloliquefaciens QLY006, and the culture medium is the balance, and the culture medium is composed of the following substances : beef extract 3g, sucrose 30g, NaCl 5g, yeast extract 4.5g, distilled water or deionized water 1000mL, the pH value of described culture medium is 6.0, and the number of viable bacteria of bacillus amyloliquefaciens QLY006 is 3.7 * 10 12 CFU / mL.

[0032] The preparation method of the above-mentioned microbial bacterial agent is to insert Bacillus amyloliquefaciens QLY006 into the culture medium for fermentation, the insertion amount is 1%, the fermentation time is 21h, the fermentation temperature is 28°C, the fermentation speed is 240r / min, and the fermentation solution The oxygen content is 70%. micro

Embodiment 3

[0033] Embodiment 3: microbial bacterial agent

[0034] The active ingredient is the thallus of the above-mentioned Bacillus amyloliquefaciens QLY006, wherein the content of Bacillus amyloliquefaciens QLY006 is 1.3% by weight, and the culture medium is the balance, and the culture medium is composed of the following materials: beef extract 4g, sucrose 35g, NaCl 6g, 5g of yeast extract, 1000mL of distilled water or deionized water, the pH value of the medium is 6.5, and the number of viable bacteria of Bacillus amyloliquefaciens QLY006 is 4.67×10 11 CFU / mL.

[0035] The preparation method of the above-mentioned microbial bacterial agent is to insert Bacillus amyloliquefaciens QLY006 into the culture medium for fermentation, the insertion amount is 1.3%, the fermentation time is 24h, the fermentation temperature is 32°C, the fermentation speed is 270r / min, and the fermentation solution The oxygen content is 75%.

[0036] The microbial bacterial agents of Examples 1-3 can be us...

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Abstract

The invention discloses an alpine grassland grass endophytic bacteria bacillus amyloliquefaciens strain, a microbial agent and a preparation method and application thereof, and belongs to the field of microorganisms, and solves the problem that existing chemical pesticides are low in environmental protection property, easy to generate drug resistance and low in safety. The bacillus amyloliquefaciens QLY006, CGMCC No.13049, is used for separating alpine grassland stipa capillata in vivo under extreme environment conditions, has a capacity of inhibiting pathogenic bacteria, and inhibits pseudomonas syringa pv.tomato, the anti-bacteria bandwidth reaches more than 0.65 cm, 65% of bacteriostat is reached for Botrytis cinerea, the microbial agent is multifunctional, the defect that chemical pesticides are low in environmental protection property, easy to generate drug resistance and low in safety in the prior can be overcome, and the microbial agent has the advantages of good environmental protection property, difficulty in generating drug resistance and high safety.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an endophytic bacillus amyloliquefaciens strain, bacterial agent, preparation method and application thereof. Background technique [0002] With the development of society, people have higher and higher requirements for food safety. However, the environmental pollution and pesticide residues caused by chemical pesticides have become a research hotspot in the biological control of plant diseases, especially endophytic bacteria in plants. The study of antagonistic bacteria has attracted the attention of many scholars. Plant endophytic bacteria refer to bacteria that live in plants but do not cause any substantial damage to plants. They have a variety of biological functions, especially the antibacterial function, making them a strong support for biocontrol bacteria resources. In addition to directly acting on pathogenic bacteria by secreting antibacterial substances and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C05F11/08C12R1/07
CPCA01N63/10C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 杨成德陈秀蓉薛莉冯中红王玉琴王颖姚玉玲崔月贞
Owner GANSU AGRI UNIV