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Method for efficiently culturing synechococcus 7002 on large scale

A high-efficiency technology for Synechococcus, which is applied in the field of large-scale and high-efficiency cultivation of Synechococcus 7002, can solve the problems of being easily affected by bacterial contamination, failure to achieve high density, and high cultivation costs, and achieve economical convenience, easy control, and cultivation high efficiency effect

Active Publication Date: 2017-06-13
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1) Unlike microalgae such as spirulina and chlorella, Synechococcus 7002 is highly sensitive to external bacterial contamination and is easily affected by bacterial contamination; 2) Synechococcus 7002 at different growth stages has different requirements for culture conditions , inappropriate light and carbon supply can easily inhibit its growth; 3) Although the division speed is fast, the efficiency of large-scale culture is low, and a high density cannot be achieved in a short period of time, resulting in high culture costs

Method used

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  • Method for efficiently culturing synechococcus 7002 on large scale
  • Method for efficiently culturing synechococcus 7002 on large scale

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Effect test

Embodiment 1

[0027] A method for large-scale and high-efficiency cultivation of Synechococcus sp. 7002, comprising the following steps (the cultivation process is as follows: figure 2 shown):

[0028] (1) First, inoculate the seed solution, the initial inoculation density value OD 750 0.10, the culture volume of each culture bag is 15 L, vitamin B in the culture medium 12 The concentration was increased to 10 µg / L, and the control light intensity was 20 µmol / (m 2 • s), the carbon dioxide concentration is 0.5%, and the ventilation volume is 45 L / h;

[0029] (2) Secondly, monitor the growth of Synechococcus 7002 in real time, when its density reaches OD 750 When it is 0.4, adjust the light intensity to 40 µmol / (m 2 • s), the carbon dioxide concentration is 0.7%, and the ventilation volume is 60 L / h;

[0030] (3) When the density of Synechococcus 7002 reaches OD 750 When it is 1.0, adjust the light intensity to 80 µmol / (m 2 • s), the concentration of carbon dioxide is 0.8%, and th...

Embodiment 2

[0037] A method for large-scale and high-efficiency cultivation of Synechococcus sp. 7002, comprising the following steps (the cultivation process is as follows: figure 2 shown):

[0038] (1) First, inoculate the seed solution, the initial inoculation density value OD 750 0.12, the culture volume of each culture bag is 18 L, vitamin B in the culture medium 12 The concentration was increased to 20 µg / L, and the control light intensity was 25 µmol / (m 2 • s), the carbon dioxide concentration is 0.5%, and the ventilation volume is 55 L / h;

[0039] (2) Secondly, monitor the growth of Synechococcus 7002 in real time, when its density reaches OD 750 When it is 0.5, adjust the light intensity to 50 µmol / (m 2 • s), the carbon dioxide concentration is 0.7%, and the ventilation volume is 70 L / h;

[0040] (3) When the density of Synechococcus 7002 reaches OD 750 When it is 1.2, adjust the light intensity to 100 µmol / (m 2 • s), the concentration of carbon dioxide is 0.9%, and t...

Embodiment 3

[0047] A method for large-scale and high-efficiency cultivation of Synechococcus sp. 7002, comprising the following steps (the cultivation process is as follows: figure 2 shown):

[0048] (1) First, inoculate the seed solution, the initial inoculation density value OD 750 0.15, the culture volume of each culture bag is 20 L, vitamin B in the culture medium 12 The concentration was increased to 40 µg / L, and the control light intensity was 30 µmol / (m 2 • s), the carbon dioxide concentration is 0.6%, and the ventilation volume is 60 L / h;

[0049] (2) Secondly, monitor the growth of Synechococcus 7002 in real time, when its density reaches OD 750 When it is 0.6, adjust the light intensity to 60 µmol / (m 2 • s), the carbon dioxide concentration is 0.8%, and the ventilation volume is 80 L / h;

[0050] (3) When the density of Synechococcus 7002 reaches OD 750 When it is 1.5, adjust the light intensity to 120 µmol / (m 2 • s), the concentration of carbon dioxide is 1.0%, and t...

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Abstract

The invention discloses a method for efficiently culturing synechococcus 7002 on a large scale. A suspended culture bag culture mode is adopted, the illumination intensity, the CO2 concentration and the ventilator capacity are regulated step by step according to the growth state of the synechococcus 7002, and vitamin B12 and iron ions are added to promote growth. The method is applicable to indoor or outdoor culture of the synechococcus. Obviously, the synechococcus 7002 can be efficiently fermented on a large scale, the efficiency is fully improved, the cost is saved, and the method is an effective method for producing synechococcus 7002 algae powder and added fermentation products. A culture bag is adopted for culture, can provide a relatively closed culture space, has the better culture effect, is economical and convenient, facilitates condition control and can be reused.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, and in particular relates to a method for large-scale and high-efficiency cultivation of Synechococcus algae 7002. Background technique [0002] Microalgae are considered to be the most promising raw materials for sustainable production of food, feed, chemical materials and fuels. Microalgae has high nutritional value and is rich in pigments, proteins, lipids, and various mineral elements and vitamins. Synechococcus is one of the two most important cyanobacteria in the ocean. It is the dominant component of the marine phytoplankton community and contributes 16.7% to the primary productivity of the ocean. It plays an important role in the photosynthesis, carbon cycle and food chain of the marine ecosystem. pivotal role. Synechococcus 7002 is a model microalgae in the sea, and it is also the fastest dividing cyanobacteria found so far. It has a natural exogenous DNA transformation system, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 曾名湧高风正吴浩浩黄敏冯广鑫
Owner OCEAN UNIV OF CHINA