Endophytic fungus of associated mangrove plant pluchea indica, application of endophytic fungus and method for enriching selenium
A technology of mangrove plants and endophytic fungi, which is applied in the field of selenium enrichment of endophytic fungi, can solve the problem of undetected acute toxicity of chrysanthemum, and achieve the effect of simple method
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[0044] Preparation of liquid seeds: add 5-8ml of sterile water to the slant culture medium covered with bacteria, scrape off mycelium, and use a rubber dropper to draw an appropriate amount of mixed liquid (mycelia + sterile water) into the seed medium medium, 30°C, 150r / min shaker culture for 48h, the mycelium overgrown the shaker flask for later use.
[0045] Selenium-enriched culture: Add different volumes (10-50) of selenium standard solutions to the shake flasks covered with bacteria to achieve the purpose of controlling the selenium concentration. Place it at 26°C and culture it in a shaker at 150r / min for 12-60h.
[0046] 1.5 Determination method of selenium content
[0047] 1.5.1 Determination of biomass
[0048]After the selenium enrichment time is over, filter the mycelia with filter paper, wash with distilled water for 3 to 5 times, scrape the mycelium into a petri dish that has been dried to a constant weight, dry it at 60°C to a constant weight, and weigh it. T...
Embodiment 1
[0091] A kind of endophytic fungus of half-lived mangrove plant Broadbacteria, which is made by the following method:
[0092] Step 1. Take the half-lived mangrove plant Broadbractia in Maoweihai, Qinzhou City, Guangxi, as the half-lived mangrove plant Broadbract endophytic fungus isolation and culture object, wherein there are roots, stems, leaves, flowers, etc.;
[0093] Step 2. Clean the roots, stems, leaves, and flowers with sterile water, dry them, and put them into an ultra-clean bench for ultraviolet sterilization. The ultraviolet sterilization generally does not exceed 150°C;
[0094] Step 3: Cut the ultraviolet-sterilized roots, stems, leaves, and flowers into small sections no larger than 1 cm, wash the small sections with sterile water, and then soak them in 200 mg / L sodium hypochlorite solution for 3-5 seconds. Fractional 75% ethanol cleaning, finally using sterile water after cleaning, using sterilized filter paper to dry the water on the surface of the half-lived...
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