High-throughput screening method for antibacterial drugs in milk

A screening method and antibacterial technology are applied in the field of antibacterial drug residue detection in milk to achieve the effect of simple and fast sample pretreatment method

Inactive Publication Date: 2017-06-13
HUAZHONG AGRI UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to provide a high-throughput screening method for antibacterial drug residues in milk, in order to overcome the defects of existing sample detect

Method used

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  • High-throughput screening method for antibacterial drugs in milk
  • High-throughput screening method for antibacterial drugs in milk
  • High-throughput screening method for antibacterial drugs in milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Establishment of a standard spectral library for antibacterial drug residues

[0031] (1) Pretreatment of antibacterial drugs in milk

[0032] To the milk sample, add 10 mL of extraction acetonitrile-water (90:10, V / V) and 0.5 g of Na2EDTA for extraction, rotate at 35 °C to a volume of about 0.5 mL, and dilute to 1.0 mL with mobile phase.

[0033] (2) Detection of antibacterial drugs in milk

[0034] Chromatographic conditions: HPLC column: Hypersil GoldenC 18 Chromatography column (150mm×2.1mm, 5μm); flow rate: 0.2mL / min. Mobile phase: A: acetonitrile, B: 0.1% formic acid aqueous solution; Gradient elution (t, min): t 0′ , A=5%; t 5′ , A=5%; t 10′ , A=25%; t 25′ , A=25%; t 40′ , A=50%; t 45′ , A=80%; t 50′ , A=80%; t 50.5′ , A=5%; t 60′ , A=5%.

[0035] Mass spectrometry conditions: measured by electrospray ion source, in multiple reaction monitoring mode, scanning in positive and negative ion modes at the same time, spray voltage is 5000V; sheat...

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Abstract

The invention belongs to the technical field of antibacterial drug residue detection in foods, particularly relates to a high-throughput screening method for antibacterial drugs in milk and relates to a method for detecting 120 antibacterial drug residues in milk at the same time. The detection method comprises the steps of (1) building a technology for pretreatment of antibacterial drugs in milk; (2) building a method for detecting antibacterial drugs in milk; (3) building a blank milk spectral library; (4) building a milk antibacterial drug residue spectral library; and (5) comparing the blank milk spectral library with the milk antibacterial drug residue spectral library to obtain the variety and the content of antibacterial drugs in a to-be-detected milk sample. The method for detecting antibacterial drug residues in a milk sample is simple and fast in operation, low in organic reagent dose and high in sensitivity. The variety and the content of antibacterial drugs in a milk sample can be quickly and accurately identified and the method is suitable for monitoring antibacterial drug residues in milk.

Description

technical field [0001] The invention belongs to the technical field of detection of antibacterial drug residues in milk, and in particular relates to a high-throughput screening method for antibacterial drugs in milk. The invention can perform high-throughput screening for 120 kinds of antibacterial drug residues in milk. Background technique [0002] As the material basis for the development of modern animal husbandry, veterinary medicine has played an important role in reducing the morbidity and mortality of livestock and poultry, promoting animal growth, improving animal product quality and increasing feed utilization. The use of antibacterial drugs in the feeding of dairy cows is very common. For example, various antibiotics and sulfa drugs are widely used to prevent and treat bovine mastitis and other diseases. As the public pays more and more attention to food safety, the problem of antibacterial drug residues in milk has also received more and more attention. There a...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 袁宗辉陈冬梅于洁陶燕飞谢书宇潘源虎黄玲利彭大鹏王玉莲程古月
Owner HUAZHONG AGRI UNIV
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