Method for shortening rotation cycle of watermelon to prevent and control fusarium wilt of watermelon
A watermelon fusarium wilt, watermelon technology is applied in the field of shortening the watermelon rotation period to prevent and control watermelon fusarium wilt, can solve the problems of small applicable area, crop phytotoxicity, instability, etc., and achieves the effects of increasing income, simple process and easy operation.
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Embodiment 1
[0023] Example 1 Inhibitory test of melon on mycelia growth of Fusarium wilt of watermelon
[0024] Watermelon Fusarium wilt was inoculated into a PDA plate, cultured at 28-30° C. for 5 days, and then punched with a hole punch to obtain mycelial blocks with a diameter of 4 mm for future use.
[0025] Inoculate a mycelium block with a diameter of 5 mm in the middle of the PDA plate medium, add 0.01 g, 0.015 g, 0.02 g, 0.025 g, and 0.03 g of cotton wool to the center of the petri dish (8.5 cm in diameter) respectively, and sterile The water was quickly mixed at a mass ratio of 1:1, and each treatment was repeated 3 times. At the same time, a control group (without adding cotton wool) was set up; then the half of the culture dish inoculated with the mycelium block was buckled upside down on the lid of the culture dish, and sealed with a parafilm. Sealed, cultured at a constant temperature of 28°C, after 72 hours the culture results were as follows: figure 1 As shown, the colony ...
Embodiment 2
[0028] Example 2 The killing effect of Mironon on the watermelon wilt bacteria in the soil in the facility greenhouse
[0029] The experiment was carried out in Huai'an City watermelon continuous cropping greenhouse, where watermelon has been continuously cropped for many years, and Fusarium wilt occurred seriously. The area of the greenhouse is 8 meters (width) * 40 meters (length), and the experiment is divided into two groups:
[0030] 1. The control group, without any treatment;
[0031] 2. Cotton treatment group, cotton fumigation;
[0032] Each group has 3 plots, each with an area of 22 square meters (2.2 meters wide and 10 meters long).
[0033] The test operation is as follows: in the first year, from the end of May to the middle of June, the watermelon residues planted in the spring stubble in the greenhouse were removed, and 20 kg of commercial organic fertilizer and 2 kg of NPK compound fertilizer (15-15-15 ), the soil is rotary tilled 20-25 cm deep;
[0034...
Embodiment 3
[0038] Example 3 The reduction effect of planting garlic in autumn on the number of watermelon wilt bacteria in the soil after soil disinfection and the prevention and control effect on the occurrence of watermelon wilt in spring
[0039] The test was carried out in the greenhouses of the control group and the cotton wool treatment group in Example 2.
[0040] In mid-to-late September, garlic was planted and managed by conventional methods in the control group and in the plots that were air-dried for 3-4 days after cotton-long disinfection treatment (for specific management methods, please refer to the literature "Planting and Management of Garlic", Li Chunmei, Farmers Get Rich Friends, 2016).
[0041] Garlic seedlings are harvested from the end of January to the middle and late February of the second year. After the garlic harvest is over, soil samples are collected in each plot according to the S-type soil extraction method, and the selective medium is used to count the bact...
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