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A method for preparing peptide calcium from fish bone

A technology of fish bone and peptide calcium, which is applied in the field of preparation of peptide calcium products, can solve problems such as fish bone softening, achieve the effects of promoting dissolution, improving product quality, and increasing utilization value

Active Publication Date: 2020-05-22
JIANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the key technical problems are the softening of fish bones, the preparation of peptides and the chelation of calcium peptides.

Method used

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  • A method for preparing peptide calcium from fish bone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Subcritical water treatment fish bones

[0031] (1) Mixing fish bones with hydrochloric acid solution: mix fish bones with hydrochloric acid solution with a pH value of 2.0 at a solid-to-liquid ratio of 1:10;

[0032] (3) Subcritical water treatment: subcritical water treatment was performed on the fish bone and hydrochloric acid mixture, the subcritical water temperature was set at 120 °C, and the treatment time was 2.0 h.

[0033] 2. Dynamic high-pressure micro-jet ultra-fine fishbone

[0034] The colloid mill was used to treat the fishbone mixture three times, and then a 40 MPa homogenizer was used to treat it twice, and then the dynamic high-pressure micro-jet was used for ultra-fine treatment. The micro-jet pressure was set at 140 MPa, and the number of treatments was 4 times.

[0035] 3. Enzymatic hydrolysis of fish bones with protease

[0036] (1) Pepsin enzymatic hydrolysis of fish bones: Pepsin was used to enzymatically hydrolyze fish bones treated with dy...

Embodiment 2

[0044] 1. Subcritical water treatment fish bones

[0045] (1) Mixing fish bones with hydrochloric acid solution: mix fish bones with hydrochloric acid solution with a pH value of 2.5 at a solid-to-liquid ratio of 1:10;

[0046] (3) Subcritical water treatment: Subcritical water treatment was performed on the mixture of fish bone and hydrochloric acid. The subcritical water temperature was set at 125 °C and the treatment time was 1.5 h.

[0047] 2. Dynamic high-pressure micro-jet ultra-fine fishbone

[0048] The colloid mill was used to treat the fishbone mixture twice, and then a homogenizer of 10-40 MPa was used to treat it twice, and then the dynamic high-pressure micro-jet was used for ultra-fine treatment. The micro-jet pressure was set at 160 MPa, and the number of treatments was 2. Second-rate.

[0049] 3. Enzymatic hydrolysis of fish bones with protease

[0050] (1) Pepsin enzymatic hydrolysis of fish bones: Pepsin was used to enzymatically hydrolyze fish bones treat...

Embodiment 3

[0058] 1. Subcritical water treatment fish bones

[0059] (1) Mixing fish bones with hydrochloric acid solution: mix fish bones with hydrochloric acid solution with a pH value of 2.0 at a solid-to-liquid ratio of 1:15;

[0060] (3) Subcritical water treatment: subcritical water treatment was performed on the fish bone and hydrochloric acid mixture, the subcritical water temperature was set at 130 °C, and the treatment time was 2.0 h.

[0061] 2. Dynamic high-pressure micro-jet ultra-fine fishbone

[0062] The fishbone mixture was treated twice with a colloid mill, then twice with a 30 MPa homogenizer, and then ultra-micronized with a dynamic high-pressure micro-jet. The pressure of the micro-jet was set at 180 MPa, and the number of treatments was three times.

[0063] 3. Enzymatic hydrolysis of fish bone with protease

[0064] (1) Pepsin enzymatic hydrolysis of fish bones: Pepsin was used to enzymatically hydrolyze fish bones treated with dynamic high-pressure micro-jet, the ...

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Abstract

A method for preparing peptide calcium from fish bones, which uses fish bones as raw materials, uses subcritical water to treat fish bones under acidic conditions, uses dynamic high-pressure micro-jet to ultrafine fish bones, and enzymolyzes them with pepsin and compound flavor protease Fish bones, and then use microwave technology to process the fish bone enzymatic hydrolyzate, and finally spray-dry to make peptide calcium products. The invention adopts subcritical water technology to treat the mixture of fish bones and hydrochloric acid, which can effectively shorten the softening speed of fish bones and promote the dissolution of calcium ions; high-pressure micro-jet treatment can greatly reduce the particles of fish bones and improve the enzyme activity of subsequent fish bones hydrolysis efficiency; using pepsin and compound flavor protease to enzymatically hydrolyze the ultrafine fish bone can promote the transformation of protein into polypeptide and form a mixture of peptide and calcium; microwave heating can promote the chelation efficiency of polypeptide and calcium, Improve product quality; the invention can achieve the purpose of simultaneously utilizing protein and calcium in fish bones, and improves the utilization value of fish bones, a by-product of surimi processing.

Description

technical field [0001] The invention relates to a method for preparing peptide calcium by using fish bone as a raw material, combined with subcritical water technology, dynamic high-pressure micro-jet treatment, protease enzymolysis, microwave treatment and spray drying, and belongs to the field of preparation of peptide calcium products. Background technique [0002] Fish is one of the main sources of protein ingested by humans, and it has the characteristics of low price, green and safe. With the improvement of people's living standards and the acceleration of the pace of life, more and more consumers love convenient, quick, green and nutritious food. Among them, surimi products are a new type of fish product developed in recent years. The raw material for surimi processing is fish meat, and the remaining fish bones after harvesting are discarded or simply crushed and used as feed, resulting in waste of resources and environmental pollution. [0003] Fish bones are rich i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/165A23L33/18A23L5/30
CPCA23V2002/00A23V2200/30A23V2250/1578A23V2250/55A23V2300/24
Inventor 沙小梅涂宗财李鑫黄涛张露王辉
Owner JIANGXI NORMAL UNIV
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