High efficiency and multi-stage supercritical CO2 extraction method for ganoderma lucidum spore oil

Abstract

The invention relates to the technical field of Chinese herbal component extraction, in particular to a microwave enhanced supercritical CO2 extraction method for ganoderma lucidum spore oil, and the method adopts ganoderma lucidum spore as the raw material, and conducts superfine grinding and bio-enzyme method combined wall breaking. The superfine grinding and bio-enzyme method combined wall breaking technology adopted by the invention not only enhances the wall breaking rate, but also improves the wall breaking efficiency by 30%-50% than that of single wall breaking method, and the wall breaking rate reaches more than 99.7%. The adopted microwave enhanced supercritical CO2 extraction can complete extraction under a mild unvironment, the operation is simple and the cost is low. superfine grinding and bio-enzyme method combined wall breaking, wet granulation and microwave enhanced supercritical CO2 extraction are carried out on the ganoderma lucidum spore, the obtained ganoderma lucidum spore oil has an oil yield of 41.3%, which is 17.1% higher than that of supercritical CO2 extraction, and the content of ganoderma triterpene, ergosterol and other bioactive constituents can be increased by 10%-20%.

Description

technical field

[0001] The invention relates to the technical field of extraction of traditional Chinese medicine components, in particular to a method for preparing ganoderma lucidum spore oil by using ganoderma lucidum spores as raw materials through ultrafine pulverization and bioenzyme combined wall breaking and microwave-enhanced supercritical CO2 extraction. Background technique

[0002] Ganoderma lucidum has a very long history as a medicine. As early as the Eastern Han Dynasty, my country’s first medicinal book "Shen Nong’s Herbal Classic" recorded: Ganoderma lucidum has six kinds of purple, red, green, yellow, white, and black, and its nature and taste are sweet and flat. It is mainly used to treat "the knot in the chest, benefit the heart, nourish the middle, increase wisdom, not forget, eat for a long time, lighten the body and not grow old, prolong life and immortality", and list Ganoderma lucidum as the top grade. The annual edition of "Pharmacopoeia of the Peopl...