Method for rapidly identifying and screening salt-tolerant materials in sorghum germination period

Abstract

The invention provides a method for rapidly identifying and screening salt-tolerant materials in the sorghum germination period. The method comprises the steps of seed treatment, root culture, sodium chloride stress treatment, salt tolerance judgment and the like. The bending degree of a root is observed. If root bending length is larger than 0.2 cm compared with that before treatment, that is, the root bends under the salt stress, the root has strong salt tolerance. If the root is upright and no change occurs after the salt treatment, it indicates that the root of the variety does not grow under the salt stress and the variety belongs to salt-sensitive sorghum materials. The method has the advantages of fast identification, accurate and visual effect, low requirement for identification environment conditions and high controllability. The method can also be used for quickly screening the limit concentration of salt tolerance of some or certain sorghum varieties, and screening salt-tolerant and salt-sensitive materials from a large number of sorghum germplasm resources or breeding materials.

Description

technical field [0001] The invention relates to the field of screening and identification of germplasm resources, in particular to a method for screening and identification of sorghum germination salt tolerance. Background technique [0002] Sorghum is the fifth most important crop in the world and has multiple uses such as grain, feed, and brewing. Due to its small genome and the completion of genome sequencing, sorghum has become another important food and energy model crop after rice. [0003] There are more than 1.5 billion mu of saline-alkali land in my country, most of which are in a barren state. Sorghum is a moderately salt-tolerant crop. Planting sorghum on medium-slightly saline-alkaline land is conducive to making full use of my country's land resources and alleviating the country's energy and feed shortages. However, due to the large differences in salt tolerance among sorghum varieties, not all varieties can obtain higher yields when planted on saline-alkali s...

AI Technical Summary

Problems solved by technology

However, for screening a large number of mutants, germplasm resources, and offspring breeding materials, this method has the following disadvantages: the identification time is long and takes 7 days; the identification results need statistical comparison, and the results are not intuitive; The root growth is messy, and the growth status of the root cannot be effectively identified

Although there are many methods for identification and screening of sorghum salt tolerance during germination, there is no relevant research on the method of screening sorghum salt-tolerant materials by using whether the roots of sorghum seeds are bent or not

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