Method for separating and preparing hydroxytyrosol from olive fruit water

A technology of olive fruit and hydroxytyrosol, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as quality and safety reduction, and achieve the purpose of ensuring safety, improving extraction rate and shortening time. Effect

Inactive Publication Date: 2017-06-23
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent application number 200710057290.3 uses olive extract as raw material to prepare hydroxytyrosol. In the whole process, not only macroporous resin is used to enrich and purify, but also concentrated at high temperature, which reduces the quality and safety of the final product.

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  • Method for separating and preparing hydroxytyrosol from olive fruit water

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) Separating the waste water left when the olive oil is pressed through a tubular centrifuge to obtain a centrifuged liquid. Take 100mL of the centrifuged liquid of olive fruit water and place it in a rotary evaporator for concentration, and concentrate it to 75mL at a temperature lower than 55°C.

[0041] 2) The above-mentioned concentrated solution was left to stand, and after cooling to room temperature, 75 mL of ethyl acetate was added and stirred, and 2 g of sodium sulfate was added while stirring.

[0042] 3) Stir continuously for 20 minutes, place in a separatory funnel and let stand for 30 minutes, it can be clearly observed that the above solution is divided into two phases.

[0043] 4) Concentrate the upper phase liquid at a temperature lower than 55° C., and obtain a hydroxytyrosol extract after microwave drying.

[0044] The purity of the hydroxytyrosol extracted from the olive fruit water by the above method is 17%, and the transfer rate is 93%.

Embodiment 2

[0046] 1) The olive fruit water is separated from solid and liquid by a disc centrifuge to obtain centrifuged liquid. Take 1L of olive fruit water and centrifuge the liquid to concentrate, and concentrate to 600mL at a temperature lower than 60°C.

[0047] 2) The above-mentioned concentrated solution was left to stand, and after cooling to room temperature, 500 mL of ethyl acetate was added, and 32 g of sodium sulfate was added while stirring.

[0048] 3) Stir continuously for 30 minutes, place in a separatory funnel and let stand for 30 minutes, it can be clearly observed that the above solution is divided into two phases.

[0049] 4) Concentrate the upper phase liquid at a temperature lower than 55° C., freeze-dry to obtain hydroxytyrosol.

[0050]The purity of the hydroxytyrosol extract extracted from the olive fruit water by the above method is 26%, and the transfer rate is 87%.

Embodiment 3

[0052] 1) Pass 1000L of olive fruit water through a coupling separation device of a tubular centrifuge and a ceramic membrane, and the obtained liquid is concentrated in a double-effect concentration tank, and concentrated to 750L at a temperature lower than 55°C.

[0053] 2) Pour the above-mentioned concentrated solution into an alcohol precipitation tank, add 750L of ethyl acetate after cooling to room temperature, start stirring, and add 27kg of industrial sodium sulfate in batches while stirring.

[0054] 3) Continue to stir for another 20 minutes and let stand for 30 minutes. It can be clearly seen from the visual mirror that the solution is divided into upper, middle and lower phases.

[0055] 4) Pour the upper phase liquid into a double-effect concentration tank, concentrate at a temperature lower than 55°C to a density of 1.2, and microwave dry to obtain a hydroxytyrosol extract.

[0056] The purity of the hydroxytyrosol extract extracted from the olive fruit water by ...

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Abstract

The invention discloses a method for separating and preparing hydroxytyrosol from olive fruit water. According to the method, a great deal of waste fruit water generated while olive oil is squeezed from olive fruit is adopted as a raw material, and a hydroxytyrosol extract is prepared by using integral techniques such as centrifugation, extraction, low-temperature concentration and drying. Strong acid or alkali and high temperature are not involved in the whole production process, and enrichment and purification are implemented without chemical materials, so that the hydroxytyrosol extract can meet security requirements of food, healthcare food and special medicinal food raw materials, the purity of hydroxytyrosol of the extract is 20-50%, and thus requirements of different products can be met. The method is efficient, energy-saving, environmentally friendly, convenient, easy to industrialize and low in operation cost.

Description

technical field [0001] The invention belongs to the technical field of separation and preparation of plant extracts, in particular to a method for separation and preparation of hydroxytyrosol from olive water. Background technique [0002] The chemical name of hydroxytyrosol (hydroxytyrosol, HT) is 3,4-dihydroxyphenylethanol, which is a natural polyphenolic compound with a small molecular weight, and its monomer is a yellow-brown oily liquid. Hydroxytyrosol has the same phenolic hydroxyl group as other phenolic substances, and it also has alcoholic hydroxyl group on the ethanol chain connected with benzene ring. It is a kind of iridoid hydroxyaromatic compound. sex. Physiological studies have found that hydroxytyrosol has good antioxidant activity and can effectively scavenge free radicals and oxidation. It has a positive role in promoting the protection of the cardiovascular system, anti-cancer and anti-cancer. Therefore, in the new era of advocating great health for the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C37/00C07C37/70C07C39/11
CPCC07C37/009C07C37/685C07C39/11
Inventor 邸多隆刘建飞火婷黄新异
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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