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Cationic nanoparticles and preparation method and application

A technology of nanoparticles and cations, applied in the field of medicine, can solve the problems of discoloration, teeth containing irritating ingredients, poor antibacterial effect, etc., and achieve the effect of good compatibility, small irritation, and strong antibacterial effect

Active Publication Date: 2017-06-27
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a cationic nanoparticle and its preparation method and its application in the direction of mouthwash, which can effectively solve the problem of poor antibacterial effect of mouthwash in the prior art, containing irritating ingredients and problems that discolor teeth

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  • Cationic nanoparticles and preparation method and application
  • Cationic nanoparticles and preparation method and application
  • Cationic nanoparticles and preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A preparation method of cationic nanoparticles, comprising the following steps:

[0033] (1) Dissolve octadecylamine in chloroform so that the concentration of octadecylamine is 0.6 mg / mL;

[0034] (2) Take 0.5 mL of the solution obtained in step (1), dissolve 20 mg of PLGA in the solution, stir and mix;

[0035] (3) Take 10mL of F68 aqueous solution with a concentration of 0.5%, mix it with the solution obtained in step (2), and then perform ultrasonic treatment at a temperature of 25°C for 6 times with a time interval of 9s and each treatment time of 5s , to obtain an emulsified solution containing PLGA nanoparticles;

[0036] (4) Place the emulsified solution obtained in step (3) at 40°C for 20 minutes in a vacuum rotary evaporation to remove chloroform

[0037] , to obtain a solution of cationic nanoparticles.

[0038] Take 0.1 mL of the above-mentioned cationic nanoparticle solution, dilute it 10 times with distilled water, and measure the average particle size ...

Embodiment 2

[0041] A preparation method of cationic nanoparticles, comprising the following steps:

[0042] (1) Dissolve octadecylamine in dichloromethane so that the concentration of octadecylamine is 0.8mg / mL;

[0043] (2) Take 0.5 mL of the solution obtained in step (1), dissolve 20 mg of PLGA in the solution, stir and mix;

[0044] (3) Take 10mL of F68 aqueous solution with a concentration of 0.6%, mix it with the solution obtained in step (2), and then perform ultrasonic treatment at a temperature of 25°C for 6 times with a time interval of 9s and each treatment time of 5s , to obtain an emulsified solution containing PLGA nanoparticles;

[0045] (4) Place the emulsified solution obtained in step (3) at 25°C for 40 minutes in a vacuum rotary evaporation to remove chloroform

[0046] , to obtain a solution of cationic nanoparticles.

[0047] Take 2mL of the above-mentioned cationic nanoparticle solution and place it under the condition of 37°C for 4h, 8h, 24h, 48h and 72h respectiv...

Embodiment 3

[0051] A preparation method of cationic nanoparticles, comprising the following steps:

[0052] (1) Dissolve octadecylamine in absolute ethanol so that the concentration of octadecylamine is 1mg / mL;

[0053] (2) Take 0.5mL of the solution obtained in step (1), dissolve 15mg of PLGA in the solution, stir and mix;

[0054] (3) Take 15mL of F68 aqueous solution with a concentration of 0.2%, mix it with the solution obtained in step (2), and then perform ultrasonic treatment at a temperature of 25°C for 6 times with a time interval of 9s and each treatment time of 5s , to obtain an emulsified solution containing PLGA nanoparticles;

[0055] (4) Put the emulsified solution obtained in step (3) under the condition of 37° C. for 35 minutes in a vacuum rotary evaporation to remove chloroform to obtain a cationic nanoparticle solution.

[0056] Put Streptococcus mutans (UA159) into BHI liquid medium for subculture. When the concentration of the strain is moderate and the activity is st...

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Abstract

The invention provides cationic nanoparticles. The preparation method includes the following steps that 1, octadecylamine is dissolved in an organic solvent, so that the concentration of the octadecylamine is 0.4-1 mg / mL; 2, a PLGA is dissolved in the solution obtained in the step 1, so that the concentration of the PLGA is 10-40 mg / mL; 3, the F68 water solution having the concentration of 0.1-1% is taken and mixed with the solution obtained in the step 2, then ultrasonic treatment is performed to obtain an emulsification solution containing PLGA nanoparticles; 4, the cationic nanoparticle are obtained through emulsifying evaporation treatment. The preparation method is simple and easy to operate, the prepared product does not contain drugs, bactericidal and antibacterial effects are achieved only by utilizing the characteristics of the nanoparticles, an antibacterial action is strong, irritation is small, the cationic nanoparticles are prepared into mouth wash and have good biocompatibility with teeth, and the phenomenon that chromatosis and furthertooth color is changed is not likely to occur.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a cationic nanoparticle, a preparation method thereof and an application in the direction of mouthwash. Background technique [0002] The imbalance of oral ecological flora has become one of the main causes of common oral diseases recognized in recent years, especially the reproduction and destruction of anaerobic flora leading to the occurrence of periodontitis and other diseases. For the health maintenance of the oral environment, mouthwash has become a hot spot chosen by many people because of its convenience, quickness and strong acceptance. Chlorhexidine and Listerine ® It is currently the most recognized and used mouthwash. Its active ingredients take antibacterial effect as the main therapeutic mechanism, and has achieved good results. It has received good attention and expanded research. Discoloration and other side effects and insufficient antibacterial ef...

Claims

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Application Information

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IPC IPC(8): A61K8/84A61K8/02A61Q11/00A61K31/765A61P31/04
CPCA61K8/0283A61K8/84A61K31/765A61K2800/413A61K2800/5426A61K2800/82A61Q11/00
Inventor 彭强朱冠印路泊遥张天旭
Owner SICHUAN UNIV
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