Preparation method of chitosan-phenolic acid conjugate
A technology of chitosan and conjugates, which is applied in the field of preparation of chitosan-phenolic acid conjugates, can solve the problems of poor solubility of chitosan and instability of phenolic acids, and achieve low cost, high output, and synthetic The effect of simple process and operation
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Embodiment 1
[0020] A kind of preparation method of chitosan-vanillic acid conjugate, concrete steps are as follows:
[0021] 1. Take a three-necked flask with a clean volume of 5L, add 2L of acetic acid with a volume fraction of 2%, add 10g of chitosan and dissolve it, then add 20mL of 1mol / L H containing 1.08g of ascorbic acid dropwise. 2 o 2 , and then filled with nitrogen for 30 minutes.
[0022] 2. Dissolve 10 g of vanillic acid in 100 mL of ethanol to obtain a vanillic acid solution, add the vanillic acid solution to the reaction system in step 1, seal the flask mouth with plastic wrap, and react at 25°C for 24 hours under electromagnetic stirring.
[0023] 3. The reaction solution was dialyzed against distilled water for 72 hours with a 14000Da molecular weight cut-off membrane to remove unreacted vanillic acid, and the obtained dialysate was dried by a spray dryer (inlet temperature 140°C, outlet temperature 77°C) to obtain chitosan-vanilla acid conjugates.
Embodiment 2
[0025] A kind of preparation method of chitosan-coumaric acid conjugate, concrete steps are as follows:
[0026] 1. Take a three-necked flask with a clean volume of 5L, add 2L of acetic acid with a volume fraction of 2%, add 10g of chitosan and dissolve it, then add 20mL of 1mol / L H containing 1.08g of ascorbic acid dropwise. 2 o 2 , and then filled with nitrogen for 30 minutes.
[0027] 2. Dissolve 10 g of coumaric acid in 100 mL of ethanol to obtain a coumaric acid solution, add the coumaric acid solution to the reaction system in step 1, seal the flask mouth with plastic wrap, and react at 25°C for 24 hours under electromagnetic stirring.
[0028] 3. The reaction solution was dialyzed against distilled water for 72 hours with a 14000Da molecular weight cut-off membrane to remove unreacted coumaric acid, and the obtained dialysate was dried by a spray dryer (inlet temperature 140°C, outlet temperature 77°C) to obtain chitosan- Coumaric acid conjugates.
Embodiment 3
[0030] A kind of preparation method of chitosan-ferulic acid conjugate, concrete steps are as follows:
[0031] 1. Take a three-necked flask with a clean volume of 5L, add 2L of acetic acid with a volume fraction of 2%, add 10g of chitosan and dissolve it, then add 20mL of 1mol / L H containing 1.08g of ascorbic acid dropwise. 2 o 2 , and then filled with nitrogen for 30 minutes.
[0032] 2. Dissolve 10 g of ferulic acid in 100 mL of ethanol to obtain a ferulic acid solution, add the ferulic acid solution to the reaction system in step 1, seal the flask mouth with a plastic wrap, and react at 25°C for 24 hours under electromagnetic stirring.
[0033] 3. The reaction solution was dialyzed against distilled water for 72 hours with a 14000Da molecular weight cut-off membrane to remove unreacted ferulic acid, and the obtained dialysate was dried by a spray dryer (inlet temperature 140°C, outlet temperature 77°C) to obtain chitosan- Ferulic acid conjugates.
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