Pretreatment method before column adsorption and purification of roselle pigment extract

Abstract

The invention belongs to the technical field of food additive production, and relates to a method for pretreating a hibiscus sabdariffa linn pigment extracting solution before on-column adsorption and purification. The method is characterized by comprising the steps of adding water into dried hibiscus sabdariffa linn calyxes, and soaking for extracting pigment, wherein a solid-liquid ratio is equal to 1: (5 to 20), the extraction temperature is 15-60 DEG C, the pH value is a natural pH value, pectinase is added or not added, and immersing extraction is carried out for 0.3-6h; filtering or centrifuging the obtained extracting solution; then, filtering by using a quartz sand filter, and enabling the filter to be in series connection with a resin adsorption column ,so as to enable filtrate, flowing out of the filter, to directly enter the macroporous resin adsorption column for purifying. The method has the characteristics that quartz sand filtering operation and resin adsorbing operation are carried out synchronously, so that the time consumed by the extracting solution, which flows out of the quartz sand filter and enters the adsorption column for adsorbing, is very short, and a macromolecular substance in the solution does not block the adsorption column due to having no time to accumulate and precipitate on the absorption column. If the pectinase is added in the extraction process, most of pectin can be removed from the source by means of hydrolysis, so that the backwashing frequency of the filter is reduced; however, the production cost is increased due to the addition of the pectinase.

Description

technical field [0001] The invention relates to a pretreatment method before column adsorption and purification of a roselle pigment extract, which belongs to the technical field of food additive production, and in particular adopts a quartz sand filter to pretreat the extract, which is carried out synchronously with the subsequent macroporous resin adsorption column , which avoids the clogging of the adsorption column by the secondary precipitation during the storage of the filtrate, and is beneficial to the subsequent resin adsorption purification operation of the pigment solution. Background technique [0002] Roselle (Hibiscus Sabdariffa Linn), also known as mountain eggplant and roselle, is an annual herb or perennial shrub of the Malvaceae Hibiscus genus, which grows in tropical and subtropical regions. The calyx is rich in anthocyanins, vitamin C, organic acids, flavonoids and polyphenols, which can be used to produce soft drinks, roselle tea, jams, fruit juices, natu...

AI Technical Summary

Problems solved by technology

After the extract is filtered or centrifuged, it is poured into the head tank, and then flows into the resin adsorption column for adsorption and purification. The macromolecular substances such as pectin, protein and cellulose in the head tank stock solution will continue to aggregate with time, resulting in secondary Secondary precipitation, resulting in blockage of the resin bed, so that the adsorption can not be carried out normally

Before the end of adsorption and desorption, it is necessary to spend a large amount of water to wash and remove the sediment in the bed, which not only wastes water, but also takes time, and the sediment is not easy to wash, and it is easy to transfer to the final product, so that the product purity decreased, the solubility decreased, and the product quality could not meet the requirements

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