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Culture medium for producing xylitol through xylose mother liquor fermentation with candida tropicalis and fermentation method

A technology of Candida tropicalis and xylose mother liquor, applied in the field of microbial fermentation, can solve the problem of high cost of xylose, and achieve the effects of low cost, improved stability and simple ingredients

Inactive Publication Date: 2017-06-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Xylose mother liquor is the concentrated waste liquid left after extracting crude xylose, which contains a certain amount of xylose, glucose and arabinose, etc., but the cost of separating and purifying xylose from it is relatively high

Method used

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  • Culture medium for producing xylitol through xylose mother liquor fermentation with candida tropicalis and fermentation method

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: the influence of medium initial pH on yield

[0028] Using the same initial fermentation medium (xylose mother liquor 200g / L, corn steep liquor 5g / L), 30 ℃, rotating speed 200r / min, the initial pH value is adjusted to 4.0, 5.0, 6.0 and 7.0 respectively, carries out shaking flask fermentation, The effect of different initial pH values ​​on xylitol production was investigated, and the results showed that the fermentation effect was the best at initial pH 5.0.

Embodiment 2

[0029] Embodiment 2: the influence of culture medium inoculum size on output

[0030] The same fermentation medium (xylose mother liquor 200g / L, corn steep liquor 5g / L, initial pH 5.0) was used at 30°C with a rotation speed of 200r / min. The liquid seeds were inoculated at 5%, 10%, and 15% (v / v) inoculum amounts, respectively, and the effects of different inoculum amounts on xylitol production were investigated. The results showed that when the inoculum amount was 15%, the fermentation effect was the best; compared with the inoculum amount of 5%, and 10%, the yield of xylitol increased by 38%, 26%, respectively.

Embodiment 3

[0031] Embodiment 3: the influence of culture medium fermentation temperature on yield

[0032] The same fermentation medium (xylose mother liquor 200g / L, corn steep liquor 5g / L, initial pH 5.0) was used, and the rotation speed was 200r / min. Shake flask fermentation was carried out at 30°C, 33°C, and 35°C respectively, and the effect of fermentation temperature on xylitol production was investigated. The results showed that the fermentation effect was the best at 35°C; compared with 33°C and 30°C, the yield of xylitol increased by 26% and 59% respectively.

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Abstract

The invention discloses a culture medium for producing xylitol through xylose mother liquor fermentation with candida tropicalis and a fermentation method and belongs to the technical field of microbial fermentation. The yield of xylitol is increased by changing components and fermentation conditions of the fermentation culture medium on the basis of dilute xylose mother liquor. The technological fermentation culture medium is low in cost, additional carbon source and trace elements are not required to be added, the technological control is simple, fermentation performance of strains is stable, the yield reaches 96.38 g / L, and the conversion efficiency is close to 100%.

Description

technical field [0001] The invention relates to a culture medium and a fermentation method for producing xylitol from candida tropicalis fermenting xylose mother liquor, and belongs to the technical field of microbial fermentation. Background technique [0002] Xylitol is a five-carbon sugar alcohol that exists in nature and is also an intermediate product of human metabolism. Xylitol has a wide range of uses in food, medicine, light industry and other fields: as an auxiliary therapeutic agent for diabetics, preventing dental caries, improving liver function, and regulating intestinal tract. [0003] The current industrialized xylitol production methods are mostly chemical production methods: corncobs, cottonseed hulls, bagasse and other materials rich in pentosan are subjected to acid hydrolysis, separated and purified to obtain xylose, and then hydrogenated to obtain xylitol. Xylose mother liquor is the concentrated waste liquid left after extracting crude xylose, which c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12R1/74
CPCC12P7/18
Inventor 陈献忠陈振张利华沈微樊游
Owner JIANGNAN UNIV