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An entomopathogenic nematode symbiotic bacterium capable of inhibiting potato black mole and its application

A technology of potato black mole disease and entomopathogenic nematode, which is applied in the direction of application, chemicals and bacteria for biological control, and can solve problems such as infestation, yield and quality hazards

Active Publication Date: 2020-04-21
黑龙江省农业科学院植物脱毒苗木研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the pathogen has strong environmental adaptability and can survive the winter on tubers, soil or mycelium through sclerotia on plant residues. as well as the surface of the potato tubers, thereby endangering yield and quality

Method used

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  • An entomopathogenic nematode symbiotic bacterium capable of inhibiting potato black mole and its application
  • An entomopathogenic nematode symbiotic bacterium capable of inhibiting potato black mole and its application

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specific Embodiment approach 1

[0014] Specific embodiment one: a kind of entomopathogenic nematode symbiont bacterium that suppresses potato black mole of the present embodiment, it is Xenorhabdus bovineii (Xenorhabdus bovineii) HEB01, is preserved in the common microorganism center of China Committee for Microorganism Culture Collection, The deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing. The deposit date is March 9, 2016, and the deposit number is CGMCC NO.12193.

[0015] The Xenorhabdus bovineii (Xenorhabdus bovineii) HEB01 of the present embodiment is obtained as follows:

[0016] Entomopathogenic nematodes were isolated from soil samples collected from Harbin City by trapping the wax moth; the primary symbiotic bacteria were isolated according to the following methods:

[0017] Infect the 5th instar larvae of the wax borer with the isolated entomopathogenic nematodes, after the death of the larvae, immerse them in 75% alcohol by volume for 3 seconds, then wipe ...

specific Embodiment approach 2

[0020] Embodiment 2: An entomopathogenic nematode symbiotic bacterium capable of inhibiting potato black mole in this embodiment is used for preventing and treating potato black mole.

specific Embodiment approach 3

[0021] Embodiment 3: This embodiment is different from Embodiment 2 in that it uses the bacterial liquid of entomopathogenic nematode symbiotic bacteria to prevent and treat potato black mole. Others are the same as in the second embodiment.

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Abstract

An entomopathogenic nematode symbiotic bacteria capable of inhibiting potato black nevus and applications thereof relate to an entomopathogenic nematode symbiotic bacteria and applications. The bacterium is Xenorhabdus bovineii HEB01, which is preserved in the General Microbiology Center of the China Microorganism Culture Collection and Management Committee. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. On the 9th, the deposit number was CGMCC NO.12193. For the prevention and treatment of potato black nevus. The pathogenic bacillus HEB01 of the present invention has a strong ability to inhibit the growth of the mycelium of the potato black nevus fungus, and increases with the increase of the volume concentration, and the bacteriostatic effect is remarkable. At the same time, the bacterium has the characteristics of low requirements on culture conditions, long duration of effect, environmental protection and non-toxicity, etc., which makes it have good development and application prospects.

Description

technical field [0001] The invention relates to an entomopathogenic nematode symbiotic bacterium and its application. Background technique [0002] China is a big potato planting country. Under the impetus of strategies such as potato staple food, my country's potato industry has developed rapidly. However, with the continuous expansion of the planting area, soil-borne diseases caused by many reasons, such as the imperfect crop rotation system and the imperfect supervision of virus-free seed potatoes, are common and aggravated year by year, seriously affecting the healthy development of the potato industry. [0003] Potato black mole is one of the most threatening soil-borne diseases in potato production. Symptoms such as mole and cracks can be formed on the surface of tubers, seriously affecting the quality and commerciality of potatoes (especially seed potatoes), causing huge economic losses. The pathogen Rhizoctonia solani can also cause canker in the seedling stage and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/20A01P3/00C12R1/01
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 杨帅闵凡祥高云飞王文重魏琪董学志吕典秋王晓丹王玲
Owner 黑龙江省农业科学院植物脱毒苗木研究所