A coronavirus vaccine based on dimerized receptor binding domain subunits

Abstract

The invention discloses a subunit coronavirus vaccine for dimerization-based receptor binding domains and belongs to the technical field of medicine. A baculovirus expresses RBD (receptor binding domain (E367-Y606) of MERS-CoV (middle east respiratory syndrome coronavirus) protein and RBD (R294-F515) of SARS-CoV (severe acute respiratory syndrome coronavirus) in insect cells, the RBDs may form a dimer through cysteine residue at 603 of S (spike) protein or form a dimer through cysteine residue at 512 of the S protein, and purified RBD protein dimer and monomer are used respectively to immunize Bald / c mice. The dimerized RBDs have the advantages that the defect that RBD monomers have poor immunogenicity is overcome and the generation of neutralizing antibodies in against MERS-CoV is increased greatly.

Description

technical field

[0001] The invention relates to a coronavirus vaccine based on a dimerized receptor binding region subunit, belonging to the technical field of medicine. Background technique

[0002] Coronaviruses belong to the genus Coronaviridae of the family Coronaviridae and are enveloped positive-strand RNA viruses with the largest genomes among all RNA viruses. Both animals and humans are hosts of coronaviruses. Coronaviruses mainly infect the respiratory and digestive tracts of mammals and birds. Six of these coronaviruses infect humans. Among them, the severe respiratory syndrome coronavirus (SARS-CoV), which broke out in China in 2002-2003, and the Middle East respiratory syndrome coronavirus (MERS-CoV), which broke out in 2012 and continues to this day, are the greatest threat to global public health. Coronaviruses also cause a number of serious animal diseases, particularly posing a serious threat to agricultural livestock and pets. For example, porcine transm...

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