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Strain for degrading neonicotinoid pesticides in soil and application of strain

A neonicotinoid and soil technology, applied in the restoration of polluted soil, fungi, microorganism-based methods, etc., can solve the problems of acetamiprid residue and other problems, and achieve the effect of efficient degradation and high repair ability.

Active Publication Date: 2017-07-07
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a kind of efficient broad-spectrum degrading neonicotinoid pesticide acetamiprid bacterial strain aiming at the problem of acetamiprid residue in soil

Method used

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  • Strain for degrading neonicotinoid pesticides in soil and application of strain
  • Strain for degrading neonicotinoid pesticides in soil and application of strain
  • Strain for degrading neonicotinoid pesticides in soil and application of strain

Examples

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Embodiment 1

[0036] Isolation and screening of acetamiprid-degrading strain Fusarium.sp CS-3:

[0037] Take 5 g of acetamiprid-contaminated soil samples and place them in 100 mL of enrichment medium containing 30 mg / L acetamiprid, -1Culture 7d. Use high-performance liquid chromatography (HPLC) to measure the degradation situation of enrichment solution to acetamiprid, after confirming that acetamiprid is degraded, insert in the enrichment medium containing 40mg / L acetamiprid with 5% inoculum size, Continue to enrich and measure the degradation situation, follow this method until the concentration of acetamiprid increases to 100mg / L, and pass passage 3 times.

[0038] The acetamiprid enrichment solution was serially diluted and spread on an inorganic salt plate with 200 mg / L acetamiprid as the sole carbon source, and cultured in a 30°C incubator for 5 days. Single colonies with different colonies grown on the plate were streaked on the LB plate for purification, and inoculated in the liqu...

Embodiment 2

[0041] Identification and growth characteristics of acetamiprid-degrading strain Fusarium.sp CS-3:

[0042] Fusarium.sp CS-3 grows slowly on LB plates, but grows faster on YPD plates. At 30°C, 72 hours can form large and fluffy colonies, dull, dry, rough, opaque, and the vegetative hyphae are tight Based on its 18S rDNA sequence (as shown in SEQ ID No.: 1) and physiological and biochemical characteristics, the strain CS-3 was identified as the Fusarium genus.

[0043] The optimal growth temperature of strain CS-3 is 30°C, and it can grow well at 25°C, but its growth is significantly inhibited at a higher temperature (40°C) ( Figure 2a ). Strain CS-3 grew well at pH 7.0, which was the optimum growth pH; when pH was 4, its growth was significantly inhibited ( Figure 2b ).

Embodiment 3

[0045] Degradation characteristics of acetamiprid-degrading bacteria Fusarium.sp CS-3:

[0046] Pick a single colony of strain CS-3 from the YPD plate, inoculate them in 5mL YPD liquid medium at 30°C, shaker 180r min -1 Cultivate for 48h. Then the culture solution of the bacterial strain was transferred to 100 mL of fresh liquid YPD medium, and the culture was continued for 20 h. 8000r·min -1 Centrifuge for 10 min, collect the bacteria, wash twice with sterilized inorganic salt medium, that is, the seed liquid. In the inorganic salt medium with a final concentration of acetamiprid of 100mg / L, inoculate the seed solution of strain CS-3 according to the inoculation amount of 5% v / v, and incubate at 30°C and 180r min -1 Shake the culture, take samples every 12 hours, and detect the concentration of acetamiprid by HPLC.

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Abstract

The invention discloses a strain for degrading neonicotinoid pesticides in soil. The strain is classified and named as fusarium.sp, has a strain number of CS-3 and has a collection number of CCTCC NO: M 2017137, and the collection date is March 21, 2017. The strain CS-3 disclosed by the invention is an aerobic microbe, the optimum growth temperature of the strain is 30 DEG C, the optimum growth pH value of the strain is 7.0, the strain CS-3 can completely degrade 50mg / L of acetamiprid within 96 hours, and the acetamiprid serves as a unique carbon source for growing the strain. The strain disclosed by the invention has important application value on biological treatment of soil contaminated by the neonicotinoid pesticides.

Description

technical field [0001] The invention belongs to the technical field of biological treatment, and relates to a bacterial strain for efficiently degrading neonicotinoid pesticides in soil and an application thereof. Background technique [0002] Neonicotinoid insecticides are derived from the botanical pesticide nicotine and mainly act on the acetylcholinesterase receptors of pests. Nicotine has long been used as an insecticide by people. As early as the 17th century, there was a history of using tobacco leaf powder to kill insects. Neonicotinoid insecticides are a new class of highly efficient and highly selective new insecticides following organochlorines, organophosphorus, pyrethroids, and carbamates. Due to their wide insecticidal spectrum, low dosage, and effective With novel mechanisms and other characteristics, it has developed rapidly in domestic and foreign markets. Acetamiprid is a representative species of chlorinated nicotinic insecticides, which belongs to methy...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/77
CPCB09C1/105C12R2001/77C12N1/145
Inventor 董维亮史周坤马江锋信丰学姜岷
Owner NANJING UNIV OF TECH
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