Color developing agent for detecting ascorbic acid and application thereof

A technology of ascorbic acid and color developer, applied in the fields of nanomaterials and analytical chemistry, can solve the problems of high detection limit, long reaction time, troublesome operation, etc., and achieve the effects of high sensitivity and easy preparation

Inactive Publication Date: 2017-07-11
CAPITAL NORMAL UNIVERSITY
View PDF2 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, there are problems such as high dete

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Color developing agent for detecting ascorbic acid and application thereof
  • Color developing agent for detecting ascorbic acid and application thereof
  • Color developing agent for detecting ascorbic acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] First step, preparation and characterization of precursors

[0048] (a) Preparation of hexagonal cobalt oxyhydroxide nanosheet suspension: 0.0238g CoCl 2 Put the powder in a 10mL centrifuge tube to get 10mM CoCl 2 Aqueous solution; 0.4g NaOH particles were placed in a 10mL centrifuge tube to obtain a 1.0mM NaOH solution; 6.696mL NaClO solution was placed in a 10mL centrifuge tube and diluted to 10mL with secondary water to obtain a 0.9M NaClO solution. Take 250 μL NaOH and 1 mL CoCl 2 The solution was placed in a 5 mL centrifuge tube, sonicated for 1 min, then 50 μL of NaClO solution was added, sonicated for 10 min, then centrifuged and dried to obtain dark brown hexagonal cobalt oxyhydroxide nanosheets. The cobalt oxyhydroxide nanosheets are dispersed in water to obtain a cobalt oxyhydroxide nanosheet suspension.

[0049] Characterization of CoOOH nanosheets: The CoOOH nanosheet suspension was characterized by transmission electron microscopy, and the results were a...

Embodiment 2

[0056] Utilize ultraviolet-visible spectrophotometer, with regard to the feasibility test drawing of embodiment 1 gained chromogenic agent, as image 3 As shown, (a) is to add 1mL of CoOOH nanosheet suspension and TMB aqueous solution into a 5mL centrifuge tube respectively, and after the color is stable, put it into a cuvette, and measure its ultraviolet absorption intensity; (b) is Add 1mL H to 2mL blue solution 2 o 2 UV absorption curve; (c) is adding 1mL H in 2mL blue solution 2 The ultraviolet absorption curve of O; (d) is the ultraviolet absorption curve of adding 100 μ L concentration of AA solution that is 100 μ M in the blue solution prepared by the above-mentioned same method; (e) is the ultraviolet absorption curve of adding 1 mL of TMB and the above-mentioned concentration of AA aqueous solution respectively absorption curve.

[0057] The intensity of b and c ultraviolet absorption curves at 652nm is basically the same, indicating that H 2 o 2 It has no effect...

Embodiment 3

[0059] The color developer obtained in Example 1 is made into a visual test paper, and the visual color change is used to detect AA. The specific steps are as follows:

[0060] 1) Determine the detection limit of AA:

[0061] After sequentially adding 0nM, 10nM, 50nM, 100nM, 200nM, 500nM, 1μM, 10μM, 50μM, 100μM and 200μM AA solutions to the blue aqueous solution of the chromogenic agent prepared in Example 1, the ultraviolet-visible absorption at 652nm The intensity gradually decreases, as Figure 4A shown. The change value of ultraviolet absorption intensity ΔA(A 0 -A) is plotted against the concentration of AA added, where A 0 And A is the intensity of ultraviolet-visible absorption at 652nm of the blue solution before and after adding AA, such as Figure 4B shown. The inset is the linear relationship between the change of UV absorption and the concentration of AA, the linear range is 10nM-1μM, and the linear equation is A 0 -A=0.19296C+0.01428, the correlation coeffici...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
Sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a color developing agent and a preparation method and an application thereof. The color developing agent is prepared by mixing a CoOOH nano sheet suspension with 3, 3', 5,5'-tetramethyl benzidine water solution according to the molar ratio CoOOH nano sheets to 3, 3', 5,5'-tetramethyl benzidine of 2:1. The color developing agent is used for detecting ascorbic acid, and has high sensitivity and low detection limit.

Description

technical field [0001] The invention belongs to the field of nanometer materials and analytical chemistry, and relates to an optical detection method of ascorbic acid. Background technique [0002] Ascorbic acid (AA) is one of the most important neurochemical substances in the brain system, and some diseases are closely related to the level of AA. Therefore, it is necessary to develop a highly selective, rapid and sensitive analytical method for the detection of AA. [0003] At present, the determination methods of ascorbic acid content include fluorescence photometry, direct iodometric method, ultraviolet spectrophotometry, kinetic photometry, indirect photometry, chemiluminescence, electrode catalytic oxidation method, high performance liquid chromatography and so on. Among them, the detection of ascorbic acid by ultraviolet-visible photometry has the advantages of large detection range, high accuracy, rapidity, simplicity, and small error. However, there are problems suc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 林雨青丁永奇李博王超赵旭
Owner CAPITAL NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap