A kind of preparation method of sericin/glycerin blend film and its product and application

A technology of blending film and glycerin, which is applied in the field of biomaterials, can solve the problems of low toxicity and biocompatibility, and achieve the effects of promoting healing, strong antibacterial and growth inhibition

Active Publication Date: 2019-06-21
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the introduction of chemical cross-linking agents will bring toxicity and low biocompatibility problems

Method used

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  • A kind of preparation method of sericin/glycerin blend film and its product and application
  • A kind of preparation method of sericin/glycerin blend film and its product and application
  • A kind of preparation method of sericin/glycerin blend film and its product and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of sericin / glycerin blend film, comprising the steps of:

[0030] (1) Preparation of sericin solution: cut silkworm cocoons into about 1cm 2 Put the fragments in a beaker, add deionized water according to 1g silkworm cocoons / 20ml water, filter and remove insoluble silk fibroin at 121°C, 0.1Mpa high temperature and high pressure for 30min, collect the filtrate, freeze-dry to obtain sericin powder; according to a certain proportion Take sericin powder and add deionized water to boil and dissolve to obtain a sericin solution with a mass concentration of 5%;

[0031](2) Preparation of sericin / glycerol blend membrane: add 20 μl, 40 μl, 60 μl, 80 μl, 100 μl of glycerin (density 1.25 g / ml) to the tanks containing 4.98ml, 4.96ml, 4.94ml, 4.92ml and 4.90 ml of a centrifuge tube with a mass concentration of 5% sericin solution, mix evenly, pour it into a mold, and dry it in a 65°C oven to form a film. The mass ratios of glycerol to sericin are 10%, 20%, 30%,...

Embodiment 2

[0037] The preparation method of nano silver / sericin / glycerol blend film comprises the steps:

[0038] Prepare a silver nitrate solution with a concentration of 50mM, cut the mixed film with a mass ratio of glycerol to sericin of 40% into small pieces, immerse it in the silver nitrate solution prepared now, and irradiate it with 365nm ultraviolet light for 20min, 40min, and 60min respectively. After drying at room temperature, nano-silver / sericin / glycerin blend films with different radiation reduction times were obtained.

[0039] In this embodiment, the purpose of the invention can be achieved when the concentration of the silver nitrate solution is 20-50 mM.

[0040] Scanning electron microscopy (SEM) (JCM-5000, JEOL, Tokyo, Japan) was used to observe the surface morphology of sericin / glycerin blend film and nano-silver / sericin / glycerol blend film to confirm the presence of nano-silver in sericin. Surface in situ synthesis of / glycerol blend membranes. The surface of the s...

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Abstract

The invention relates to a preparation method of a sericin / glycerol blend membrane as well as a production and application thereof. The preparation method comprises the following steps: mixing sericin solution with glycerol, then pouring into a mould, drying, and carrying out film formation, so that the sericin / glycerol blend membrane is obtained. The defects of brittleness and difficulty in forming of sericin can be overcome by utilizing the glycerol, and flexibility of the sericin membrane is improved, so that the sericin membrane can be mildly and softly contacted with skin; then in-situ synthesis of nanosilver is carried out on the surface of the sericin / glycerol blend membrane by virtue of a green simple method, so that a nanosilver / sericin / glycerol blend membrane with antibacterial effect is prepared, and the nanosilver / sericin / glycerol blend membrane has excellent shape, stability and mechanical properties; and meanwhile, the sericin / glycerol blend membrane has good biocompatibility and has important economic benefit and social significance.

Description

technical field [0001] The invention belongs to the field of biomaterials, and in particular relates to a method for preparing a sericin / glycerin blend film, and also relates to products and applications prepared by the method. Background technique [0002] Sericin and fibroin are the main components of silk protein, and sericin accounts for 25%-30% of the total silk protein. Sericin has 18 kinds of amino acids, most of which have polar groups such as hydroxyl, carboxyl, and amino groups. These polar hydrophilic groups endow sericin with good moisture retention, hygroscopicity and reactivity. Sericin is a natural, readily available, and inexpensive protein resource. The high hydrophilicity of sericin can maintain the moist environment of the wound and absorb the wound exudate, which in turn contributes to the healing of the wound. Sericin can resist oxidation, anticoagulation, and has good biocompatibility, reactivity and biodegradability, so sericin is considered to be an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L89/00C08K5/053C08K3/08C08J5/18
CPCC08J5/18C08J2389/00C08K3/08C08K5/053C08K2003/0806C08K2201/011C08L89/00
Inventor 何华伟杨梅蓉王叶菁蔡蕊陶刚赵萍夏庆友
Owner SOUTHWEST UNIV
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