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Compound microbial flora for degradation of ammonia nitrogen

A technology of compound microbial bacteria and ammonia nitrogen is applied in the field of ammonia nitrogen degradation, which can solve the problems of difficulty in ammonia nitrogen degradation, and achieve the effect of solving ammonia nitrogen degradation and improving ammonia nitrogen degradation efficiency.

Inactive Publication Date: 2017-07-14
SHANGHAI ZHONGHAILONG NEW ENERGY
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Problems solved by technology

[0003] The purpose of the present invention is to provide a convenient microbial complex flora to solve the problem of difficult degradation of ammonia nitrogen in sewage under low temperature conditions

Method used

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Embodiment Construction

[0010] The present invention has determined a strain combination for the degradation of ammonia nitrogen through long-term research, and its compound degradation bacterial strain is composed of nitrifying bacteria strains Nitrosoma, Pseudomonas, Bacillus and Nitrobacter. The complex action between bacteria can effectively degrade ammonia nitrogen.

[0011] Take 5ml of the bottom sludge from the Songhua River in winter, inoculate it into 50ml screening medium, enrich at 2°C and 150rpm / min for 10 days, inoculate it on a solid medium for 5 days after gradient dilution, and select a typical single colony for purification Cultivation, the ammonia nitrogen degradation experiment was carried out on the pure culture strains obtained finally, and the strains whose ammonia nitrogen degradation rate reached more than 50% within 30 minutes were selected, and about 25 strains of 8 genera were isolated by referring to the "Common Bacteria System Identification Manual".

[0012] After mutual...

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Abstract

The invention aims to provide a microbial flora that can perform degradation of ammonia nitrogen at low temperature. The microbial flora includes Nitrosomonas, Pseudomonas, Bacillus and Nitrobacter. The microbial flora is separated and screened from active sludge and can perform degradation of ammonia nitrogen in sewage at low temperature; when bacterial strain quantity reaches 10<9>-10<11> cfu / ml and initial ammonia nitrogen concentration is 5.0 mg / l, sodium acetate, as a carbon source, is added according to that C / N ratio is 2; under the conditions that temperature is 2 DEG C, pH value is 7.2 and rotation speed is 150 rpm (dissolved oxygen is higher than 2 mg / L), the ammonia nitrogen is degraded for 30 min, wherein degradation rate can reach 9.350 mg(NH4<+>) / L*h. Compared with conventional single bacterial strain, the compound microbial flora can greatly increase the degradation rate of the ammonia nitrogen in sewage. The invention provides a high-quality and high-effective bacterial strain for sewage treatment.

Description

technical field [0001] The invention relates to a composite microbial flora, which belongs to the field of microorganisms and relates to the degradation of ammonia nitrogen in the process of sewage treatment. Background technique [0002] At present, the main process of China's sewage treatment plants and sewage treatment equipment is biological treatment technology, which creates good environmental conditions for microorganisms and enables microorganisms to effectively degrade harmful substances in sewage. Temperature affects the physiological activities of microorganisms to a large extent, thus affecting the effect of sewage treatment. According to investigations, the problem of sewage treatment in cold regions has seriously affected the quality of life and economic development of local people. The growth of any microorganism has a suitable temperature. Within a certain temperature range, as the temperature rises, the growth of microorganisms accelerates. When the tempera...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/38C12R1/01C02F101/16C02F103/20
CPCC02F3/34C02F2101/16C02F2103/007C02F2103/20C12N1/20
Inventor 朱恩灿陈金艳邢斌杨丽
Owner SHANGHAI ZHONGHAILONG NEW ENERGY
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