Chinese yam polysaccharide extracting method

An extraction method, a technology of yam polysaccharides, applied in the field of extraction of yam polysaccharides, can solve the problems of low extraction rate, short extraction time, and large power consumption, and achieve the effects of optimizing extraction conditions, simplifying extraction process, and improving extraction rate

Inactive Publication Date: 2017-07-18
LIAONING UNIVERSITY
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The method of hot water extraction is low in cost, takes a long time, and the extraction rate is not high; ultrasonic-assisted extraction has a short time and high efficiency, but ultrasonic may degrade soluble polysaccharides, ultrasonic equipment is expensive, and production costs are high; microwave-assisted extraction is efficient , Uniform heating, suitable for thermally stable products, but consumes a lot of power,...

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  • Chinese yam polysaccharide extracting method
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  • Chinese yam polysaccharide extracting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Determination of suitable working conditions for extracting yam polysaccharides by ultrafiltration

[0040] 1. Operation process

[0041] 1) Accurately weigh 500g of whole fresh yam, peel and cut into pieces.

[0042] 2) Add 1000ml of 1% sodium bisulfite solution with pH=8.5 according to the ratio of material to liquid (g / ml) of 1:2, and perform beating to obtain yam homogenate.

[0043] 3) The yam homogenate was centrifuged at 6000 rpm and 4°C for 20 min.

[0044] 4) Filter the supernatant to remove starch.

[0045] 5) Take the filtrate to adjust the pH=3.5, and stir it magnetically for 1 hour to obtain a suspension.

[0046] 6) Centrifuge the suspension at 6000 rpm and 4°C for 20 min to precipitate yam protein.

[0047] 7) Adjust the ultrafiltration pH of the centrifuged supernatant to a certain value, adjust the ultrafiltration pressure to a certain value, adjust the ultrafiltration temperature to a certain value, and use an ultrafiltration membrane wi...

Embodiment 2

[0060]Example 2: Response surface optimization and analysis of ultrafiltration extraction of yam polysaccharides

[0061] Design-Expert 8.0.6 software was used in the experiment for response surface design and result analysis.

[0062] (1) Experimental design results

[0063] Table 2 Results of Box-Behnken experimental design

[0064]

[0065]

[0066] (2) Regression model analysis of variance

[0067] Table 3 Regression model analysis of variance of response surface test

[0068]

[0069] In order to check the variables, ultrafiltration pH value (A), ultrafiltration temperature (B), and ultrafiltration pressure (C), the impact on the extraction rate, the Box-Behnken test design was used, and the test results are shown in Table 3. By applying multiple regression to analyze the experimental data, predict the reaction Y (extraction rate of polysaccharide) to obtain the following equation:

[0070] R 1 =39.788-2.22A+2.75B-1.44C-1.08AB+1.37AC+1.41BC-3.47A 2 +0.24B ...

Embodiment 3

[0076] Embodiment 3: the mensuration of Chinese yam polysaccharide composition

[0077] The composition of yam polysaccharide samples measured by the national standard method is shown in Table 4. The polysaccharide content is 43.84%, the protein content is 18.52%, the crude fat content is 2.52%, the crude fiber content is 1.28%, the moisture content is 9.62%, and the ash content is 4.28%.

[0078] Table 4 Polysaccharide Composition Table

[0079]

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Abstract

The invention discloses a Chinese yam polysaccharide extracting method. The method comprises the following steps: 1) taking fresh Chinese yam as a raw material, peeling, stripping and slicing; 2) adding an extracting solution, and pulping to obtain Chinese yam homogenate; 3) centrifuging the Chinese yam homogenate to obtain Chinese yam homogenate centrifugal supernatant; 4) filtering the Chinese yam homogenate centrifugal supernatant to remove starch in order to obtain filtrate; 5) adjusting the pH of the filtrate, and performing magnetic stirring to obtain a suspension; 6) centrifuging the suspension, and precipitating Chinese yam proteins; 7) adjusting the ultrafiltration pH, ultrafiltration temperature and ultrafiltration pressure intensity of the supernatant to perform ultrafiltration; 8) performing vacuum freeze drying on an intercepted part to obtain a Chinese yam polysaccharide. In the Chinese yam polysaccharide extracting method, the Chinese yam polysaccharide is extracted by an ultrafiltration concentration method, so that the polysaccharide extracting rate is up to 88.69 percent; moreover, the method is easy to operate, and is easy to implement. As proved by component measurement, infrared spectrum analysis, scanning electron microscope analysis and antioxidant activity measurement, the purity of the Chinese yam polysaccharide is 43.84 percent, and high antioxidant activity is achieved.

Description

technical field [0001] The invention belongs to the field of extracting functional biomacromolecular substances from natural raw materials to produce functional health food, and specifically relates to a method for extracting yam polysaccharides. Background technique [0002] There are more than 600 species of yam (Dioscorea genus), of which more than 50 species are commercially grown as crops around the world, contributing 200 calories of energy to about 60 million people per day in humid tropical regions. Since ancient times, yam has been considered to have the same origin of medicine and food. The first Chinese pharmacy book "Shen Nong's Materia Medica" once introduced the various medicinal effects of yam. Yam has a good effect on the prevention and treatment of diabetes, has obvious improvement on digestive system diseases, and also has positive effects on enhancing immune function and anti-aging. A large number of studies have shown that yam polysaccharide is one of th...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 薛友林陈佳男高琦李京儒彭雪
Owner LIAONING UNIVERSITY
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