Chinese yam polysaccharide extracting method

Abstract

The invention discloses a Chinese yam polysaccharide extracting method. The method comprises the following steps: 1) taking fresh Chinese yam as a raw material, peeling, stripping and slicing; 2) adding an extracting solution, and pulping to obtain Chinese yam homogenate; 3) centrifuging the Chinese yam homogenate to obtain Chinese yam homogenate centrifugal supernatant; 4) filtering the Chinese yam homogenate centrifugal supernatant to remove starch in order to obtain filtrate; 5) adjusting the pH of the filtrate, and performing magnetic stirring to obtain a suspension; 6) centrifuging the suspension, and precipitating Chinese yam proteins; 7) adjusting the ultrafiltration pH, ultrafiltration temperature and ultrafiltration pressure intensity of the supernatant to perform ultrafiltration; 8) performing vacuum freeze drying on an intercepted part to obtain a Chinese yam polysaccharide. In the Chinese yam polysaccharide extracting method, the Chinese yam polysaccharide is extracted by an ultrafiltration concentration method, so that the polysaccharide extracting rate is up to 88.69 percent; moreover, the method is easy to operate, and is easy to implement. As proved by component measurement, infrared spectrum analysis, scanning electron microscope analysis and antioxidant activity measurement, the purity of the Chinese yam polysaccharide is 43.84 percent, and high antioxidant activity is achieved.

Description

technical field [0001] The invention belongs to the field of extracting functional biomacromolecular substances from natural raw materials to produce functional health food, and specifically relates to a method for extracting yam polysaccharides. Background technique [0002] There are more than 600 species of yam (Dioscorea genus), of which more than 50 species are commercially grown as crops around the world, contributing 200 calories of energy to about 60 million people per day in humid tropical regions. Since ancient times, yam has been considered to have the same origin of medicine and food. The first Chinese pharmacy book "Shen Nong's Materia Medica" once introduced the various medicinal effects of yam. Yam has a good effect on the prevention and treatment of diabetes, has obvious improvement on digestive system diseases, and also has positive effects on enhancing immune function and anti-aging. A large number of studies have shown that yam polysaccharide is one of th...

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Problems solved by technology

The method of hot water extraction is low in cost, takes a long time, and the extraction rate is not high; ultrasonic-assisted extraction has a short time and high efficiency, but ultrasonic may degrade soluble polysaccharides, ultrasonic equipment is expensive, and production costs are high; microwave-assisted extraction is efficient , Uniform heating, suitable for thermally stable products, but consumes a lot of power, easily destroys the polysaccharide structure, and sometimes causes difficulty in extract separation. The equipment is suitable for powdery, granular and other materials, and has limitations on the state of raw materials; Enzymatic hydrolysis can effectively remove the cell wall, but because it is prone to bacterial contamination under mild conditions, and the price of enzymes is high, excessive enzymes will introduce proteins

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