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Method for succulent plant propagation through bacterium and application of bacterium

A technology for succulents and bacteria, applied in the field of plant propagation, can solve the problems of easy vitrification and growth rate of succulent plant materials, inability to establish a tissue culture system, slowness, etc., and achieve the effect of solving vitrification and slow growth rate.

Active Publication Date: 2017-08-08
CHONGQING LANDSCAPE & GARDENING RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some botanical gardens and operators in Beijing, Shanghai, Xiamen, Tianjin and other places are at the forefront in the scientific research of succulent plants, and have not yet formed a technological advantage, unable to compete with foreign succulent products
At present, although some enterprises have begun to carry out rapid propagation of succulents through tissue culture, they are unable to establish a tissue culture system due to technical problems such as easy vitrification of succulents and slow growth.

Method used

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  • Method for succulent plant propagation through bacterium and application of bacterium
  • Method for succulent plant propagation through bacterium and application of bacterium
  • Method for succulent plant propagation through bacterium and application of bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method for succulent plant propagation, comprises the steps:

[0024] (1) Sterilization and induction of explants

[0025] Put the mature seeds in a sterile Erlenmeyer flask, soak them with 75% ethanol for 30 seconds, rinse them with sterile water for 3-5 times, then soak them with 15% sodium hypochlorite solution for 20 minutes, and rinse them with sterile water for 3-5 times. The sterilized seeds were inoculated into induction medium.

[0026] The induction medium can be a conventional medium. In this example, the composition of the induction medium is: MS medium + BA0.5-2mg L -1 + agar 7g·L -1 + sucrose 30g·L -1 , pH value is 5.8. Wherein, BA is 6-benzylaminopurine.

[0027] After the sterilized seeds are cultured in the induction medium for 3 months, they gradually grow into a complete plant, and the leaves of the plant are inoculated in the subculture medium, and after 1 to 3 months, the embryogenic callus is induced. Most of the embryogenic callus was vi...

Embodiment 2

[0046] The difference from Example 1 is that in this example, the leaves collected from the mother plant are used as explants, and the leaves are pretreated before being sterilized. The specific method is as follows: the leaves collected from the mother plant are placed in In a clean, ventilated, and dry environment, after 7 days, after the petiole wound has formed a protective layer, soak the leaves in soapy water for 20 minutes at noon on consecutive sunny days, then rinse them with running water for 1 hour, and then sterilize them in an ultra-clean workbench. Bacteria, the sterilization method is the same as that of seeds. Finally, the sterilized leaves are inoculated in the induction medium, and after 1 to 3 months, embryogenic callus is formed at the wound of the leaves.

[0047] Before the leaves are sterilized, pretreatment is carried out to form a protective layer on the wounds of the petioles to prevent the leaves from rotting due to high water content. The leaves ar...

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Abstract

The invention discloses a method for succulent plant propagation through a bacterium and application of the bacterium. The bacterium is actinomycetes of Homoserinibacter gongjuensis. The propagation method comprises the following steps: (1) explant sterilization and induction: taking explant, inoculating the explant in induction medium after sterilization and inducing to generate embryogenic callus; (2) inoculation and germination: jointly inoculating the Homoserinibacter gongjuensis bacterium and the embryogenic callus in germination medium to form normal cluster bud; (3) strong seedling and transplantation: inoculating the normal cluster buds cultured in the step (2) in strong seedling medium, culturing the cluster buds into strong cluster seedlings which can be transplanted and transplanting. The method disclosed by the invention breaks sterile environment required by general plant tissue culture and effectively solves the problems of general vitrification, slow growth speed and the like in a succulent plant tissue culture process by inoculating the symbiotic bacterium of Homoserinibacter gongjuensis in the succulent plant tissue culture process.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a method for using bacteria to propagate succulent plants and the application of the bacteria. Background technique [0002] Succulents, also known as succulent plants and succulent plants, mean that at least one of the plant's vegetative organs has developed parenchyma to store water, and its appearance is plump, enlarged and juicy than ordinary plants. According to the statistics of experts from the Swiss International Succulents Association in 2012, succulents belong to 83 families, 690 genera, and about 12,500 species. There are more than 60 families and more than 10,000 species commonly cultivated in my country. Succulents were first popular in East Asian countries such as Japan and South Korea. At present, a complete and mature cultivation and screening industry has been formed in these countries. In recent years, the domestic succulent plant market has continue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 李玲莉李卿孔立生邹世慧汤丽红韦冠吾田立超
Owner CHONGQING LANDSCAPE & GARDENING RES INST
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