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Application of holarrhine B in improvement of bacteriostatic drug

A technology of antidiarrheal wood base and drug activity, applied in the field of medicine, can solve problems such as difficulty in drug-resistant bacteria and increased fatality rate

Inactive Publication Date: 2017-08-11
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason for the increase in case fatality rate is the difficulty of drug use caused by drug-resistant bacteria

Method used

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  • Application of holarrhine B in improvement of bacteriostatic drug
  • Application of holarrhine B in improvement of bacteriostatic drug
  • Application of holarrhine B in improvement of bacteriostatic drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Determination and comparison of synergistic antibacterial activity of antidiarrhealine B against methicillin-resistant Staphylococcus aureus (MRSA) in vitro

[0014] The in vitro antibacterial activity of the compound antidiarrhea B was determined with reference to the drug susceptibility test protocol of the American Committee for Clinical Laboratory Standardization CLSI (formerly NCCLS).

[0015] Materials and Methods

[0016] Experimental strain:

[0017] MRSA (methicillin-resistant Staphylococcus aureus), these strains were subcultured and used in this example.

[0018] Subculture steps:

[0019] 1. Take out the bacteria-containing glycerol tube from the -80°C refrigerator, inoculate it in the nutrient broth medium by aseptic operation, and incubate with vibration for 18-24 hours.

[0020] 2. Heat the Erlenmeyer flask containing broth agar medium until fully melted. After the culture medium was cooled to about 50°C, the aseptic method was used to invert the plat...

Embodiment 2

[0044] Embodiment 2: the preparation of tablet

[0045] Take 50g of antidiarrheal base B, add 150g of starch fine powder, mix uniformly by equal increment method, add 5g of talcum powder, 2g of magnesium stearate, mix uniformly, granulate with 85% ethanol, dry, granulate, and press into 1000 tablets, 3 times a day, 2 tablets each time.

Embodiment 3

[0046] Embodiment 3: the preparation of capsule

[0047] Take 50g of antidiarrhealine B, add 200g of fine starch powder, 10g of talcum powder, 5g of magnesium stearate, mix well, granulate, dry, put into capsules, and make 1000 capsules, 3 times a day, 2 times each time piece.

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Abstract

The invention belongs to the technical field of medicine and discloses a natural compound holarrhine B, a preparation method thereof and application of the holarrhine B in preparation of an associated bacteriostatic drug. A chemical name of the compound is the holarrhine B. An in-vitro culture method screening experiment is adopted, an in-vitro bacteriostatic activity test result proves that the holarrhine B has the remarkable in-vitro bacteriostatic activity effect when being associated with penicillin in use in an associated bacteriostatic test, and the drug resistance of drug-resistance bacteria can be remarkably reduced. The problem of the resistance of the bacteria to multiple antibiotics can be effectively solved by adopting the drug, the compound components have a remarkable synergistic effect, the antibiotic usage amount can be decreased, and accordingly the side effects and untoward effects of the antibiotics are correspondingly reduced. The holarrhine B can play the synergic bacteriostatic effect with penicillin under the concentration of 0.5-2.0 mmol / ml, so that the minimum inhibitory concentration of the penicillin to methicillin-resistant staphylococcus epidermidis is reduced to be 1 / 8 of the original.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to the application of compound antidiarrhea B in improving the activity of antibacterial drugs. Background technique [0002] Due to the over-reliance and abuse of antibiotics in recent years, the types of drug-resistant bacteria have increased rapidly, and new strains with multi-drug resistance have been rapidly derived. These multi-drug-resistant bacteria are juxtaposed with tuberculosis and HIV. Three pathogenic microorganisms that pose a major threat to human health. In the 1950s and 1960s, known as the "golden age" of antibiotics, about 7 million people died of infectious diseases worldwide every year, and this number rose to 20 million by 1999. In the United States, known as the most technologically developed country in the world, the number of deaths from infectious diseases increased by 40% between 1982 and 1992, and the number of deaths from sepsis increased by 89%. The ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/58A61K31/43A61P31/04
CPCA61K31/58A61K31/43A61K2300/00
Inventor 苏健孙博航周丽娜杨宇谢海花郭建艳连敬杰
Owner SHENYANG PHARMA UNIVERSITY
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