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ICPMS (inductively coupled plasma mass spectrometer) detection kit for detecting twenty elements in serum

A kit and serum technology, which can be used in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of high temperature heating, easy introduction of pollution, and unfavorable detection.

Inactive Publication Date: 2017-08-11
BIOYONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of microwave digestion, due to the repeated use of quartz tubes or Teflon test tubes, it is easy to introduce pollution between two digestions, which makes the detection results of trace elements higher
Moreover, during the digestion process, high temperature heating is required, and there is a possibility of element loss in serum samples
At the same time, microwave digestion is used to process serum samples. The process is cumbersome. Although the digestion time is short, after the digestion is completed, the cooling time is long, which is not conducive to the detection of large sample sizes.

Method used

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  • ICPMS (inductively coupled plasma mass spectrometer) detection kit for detecting twenty elements in serum
  • ICPMS (inductively coupled plasma mass spectrometer) detection kit for detecting twenty elements in serum
  • ICPMS (inductively coupled plasma mass spectrometer) detection kit for detecting twenty elements in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1: Selection of elemental blood collection tubes

[0106] Currently, commercially available blood collection tubes include:

[0107] No. 1: Specification Vacutainer Trace Element Serum, BD Company, model 368380.

[0108] No.2: Specification Vacutainer Serum, BD Company, model 367812.

[0109] No.3: Specification Z Serum Sep Clot Activator, Greiner Bio One Company, model 454067CN.

[0110] No. 4: Specification Z Serum Clot Activator, Greiner Bio One, model 454204.

[0111] No.5: Specification Vacutainer SST II Advance, BD Company, model 367957.

[0112] The term "trace element blood collection tube" refers to a special blood collection tube used to contain blood samples or biological tissues. Although theoretically the material of the blood collection tube should not contain any foreign elements brought in by the silicification of the tube wall, in the actual production process, due to the material, processing equipment and air environment, the introduction o...

Embodiment 2

[0151] Embodiment two: the production and detection requirements of the kit:

[0152] Preparation of standard solution:

[0153] 1 Standard material: single standard solution, calcium, magnesium, iron, copper, zinc, selenium, vanadium, chromium, manganese, cobalt, nickel, arsenic, molybdenum, cadmium, tin, antimony, barium, mercury, thallium, lead (1000mg / L, National Nonferrous Metals and Electronic Materials Analysis and Testing Center)

[0154] 2 Prepare the winning standard solution: nitric acid, purified water, existing standard solution

[0155]

[0156]

[0157] 3 Preparation of target concentration standard solution:

[0158]

[0159] The target concentration of each element in the standard solution:

[0160]

[0161] 4. Preparation of diluent: original nitric acid: ultrapure water = 2:98 (v / v), to obtain 2% nitric acid solution.

[0162] 5. The preparation method of the internal standard stock solution is: take a certain mass of indium, germanium, lute...

Embodiment 3

[0184] Example 3: ICP-MS detection of 20 elements in healthy human serum

[0185] Collect the fasting venous blood of healthy people, according to the detection procedure of the test kit in the present invention, utilize inductively coupled plasma mass spectrometer to analyze 20 kinds of elements (comprising: aluminum, vanadium, chromium, manganese, iron, cobalt, nickel, Copper, zinc, arsenic, selenium, molybdenum, cadmium, tin, antimony, barium, calcium, mercury, thallium, lead and magnesium) detection experiments:

[0186] 1 Instrument and working parameters

[0187] CLIN-ICP-QMS was used for elemental analysis. The tuning solution of the instrument was 10ppb Li, Co, In, Ba, Ce, U. The basic parameter settings of the instrument are shown in Table 6.

[0188] Table 6CLIN-ICP-QMS instrument parameter settings

[0189]

[0190] Since the collision reaction cell is obviously helpful to reduce interference and increase the detection limit, we use the helium collision cell mo...

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Abstract

The invention provides a method for determining twenty elements in serum and a matched kit. The kit is mainly used for pretreatment and quality control of required samples when the content of twenty elements in serum is determined through an ICP-MS (inductively coupled plasma mass spectrometer). The kit comprises a diluent, a standard solution containing twenty microelements, a quality control sample, an internal standard stock solution and the like. The kit is easy to use during pretreatment of the required samples, low in cost, high in accuracy and precision, good in stability and suitable for detection of clinical high-flux samples. The ICPMS detection kit can be used for detecting the content of toxic elements in human serum, trace toxin pollution to environment and trace stimulant ingredients as well as forensic toxicological detection for human bodies or corpses.

Description

technical field [0001] The invention relates to the field of element detection, and is composed of a brand-new kit for detecting elements in human serum by ICP-MS. Background technique [0002] According to the content of elements in the human body, minerals can be divided into macro elements and trace elements. The macro elements refer to elements that account for more than one ten thousandth of the body weight, including calcium (Ca), sodium (Na), phosphorus ( P), potassium (K), magnesium (Mg), chlorine (Cl) and sulfur (S), etc. The trace element refers to the content below 1 / 10,000 of the total amount of the human body, and the daily intake of trace elements is less than 100mg. In 1995, copper (Cu), cobalt (Co), chromium (Cr), iron (Fe), fluorine (F), iodine (I), manganese (Mn), molybdenum (Mo), selenium (Se ) and zinc (Zn) and other 10 elements are listed by the expert committee of the FAO / WHO international organization as indispensable essential trace elements to main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 陈文举黎庆张荣熊婵李大为马庆伟
Owner BIOYONG TECH
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