Multi-photon super-resolution micro-imaging device and method
A microscopic imaging and super-resolution technology, applied in microscopes, measuring devices, material analysis by optical means, etc., can solve the problems of high cost, complicated devices, difficult to popularize and apply, and achieve the effect of low cost and simple structure
Inactive Publication Date: 2017-08-15
王富
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Problems solved by technology
The setup is very complex due to the need to synchronize the pulses from the two lasers
More importantly, the use of two femtosecond light sources will make the cost of the device expensive and difficult to popularize and apply
Method used
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[0028] Embodiments of the technical solutions of the present invention will be described in detail below in conjunction with the accompanying drawings. The following examples are only used to illustrate the technical solutions of the present invention more clearly, and therefore are only examples, rather than limiting the protection scope of the present invention.
[0029] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application shall have the usual meanings understood by those skilled in the art to which the present invention belongs.
[0030] Figure 1 to Figure 3 The multiphoton super-resolution microscopic imaging device provided for an embodiment, the reference signs are as follows:
[0031] 1-Laser light source, 2-Half-wave plate, 3-Beam splitting device, 4-First beam expander, 5-Combining device, 6-Glass rod, 7-Pulse time modulation device, 8-Single-mode polarization maintaining fiber, 9-Second energy switch...
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The invention provides a multi-photon super-resolution micro-imaging device and a multi-photon super-resolution micro-imaging method. The multi-photon super-resolution micro-imaging device comprises a laser light source, a light splitting device, a light combining device, an objective lens and a photon detector, wherein the laser light source emits laser to the light splitting device, the light splitting device splits the incident laser into excitation laser and de-excitation laser, an optical path which the excitation laser passes is a first optical path, an optical path which the de-excitation laser passes is a second optical path, the second optical path is provided with a spatial modulation device, and the spatial modulation device is used for performing spatial modulation on the de-excitation laser, so that a light spot of the de-excitation laser becomes a light spot of a central zero point; and the light combining device is used for overlapping the excitation laser and the de-excitation laser into coaxial light rays, and the overlapped laser is used for irradiating a fluorescent sample after being focused by means of the objective lens. The multi-photon super-resolution micro-imaging device provided by the invention has the advantages of simple structure and low cost.
Description
technical field [0001] The invention relates to microscopic imaging, in particular to a multiphoton super-resolution microscopic imaging device and method. Background technique [0002] Laser confocal microscope is a new generation of microscope born with the rapid development of optics, video, computer and other technologies in the 1980s. At present, it has become a powerful research tool in the fields of morphology, molecular cell biology, neuroscience, pharmacology, and genetics. Multiphoton confocal microscopy is an imaging technique developed in recent years, mainly including two-photon microscopy and three-photon microscopy. state. Compared with traditional confocal microscopes, multiphoton confocal microscopes have the following advantages: (1) Infrared lasers with strong penetrability in biological tissues can be used as excitation light sources, which can solve the imaging problem of deep substances in biological tissues; 2) Since the wavelength of multiphoton fl...
Claims
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Login to View More IPC IPC(8): G02B21/00G01N21/64
CPCG02B21/0076G01N21/6402G01N21/6458G02B21/0068
Inventor 王富张辉乔丙闪张晓蕾
Owner 王富