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Drug-carried nanoparticle, aerogel and preparation method and application thereof

A drug-loaded nanometer and hydrogel technology, which is applied in the fields of pharmaceutical formulation, medical science, capsule delivery, etc., can solve the problems of limited application, achieve the effect of improving the lesion site, increasing the clinical cure rate, and reducing postoperative complications

Active Publication Date: 2017-08-18
ZHEJIANG PHARMA COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned tissue engineering materials are all solid, and they need to be carefully processed and shaped in actual application to be used for the repair of defective tissue, especially for the repair of tissue defects with irregular shapes, the application is limited

Method used

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  • Drug-carried nanoparticle, aerogel and preparation method and application thereof
  • Drug-carried nanoparticle, aerogel and preparation method and application thereof
  • Drug-carried nanoparticle, aerogel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0066] see figure 1 , is a schematic flow chart of the preparation method of the drug-loaded nanoparticles provided by the present invention. The preparation method of the drug-loaded nanoparticles comprises the following steps:

[0067] Step S1: Dissolve an appropriate amount of CC and 5 mg of VCM in 10 mL of water to form a solution A;

[0068] Step S2: Dissolving an appropriate amount of QAC and 1.8 mg of VCM in 8 mL of water to form solution B;

[0069] Step S3: Add solution B dropwise to solution A, and prepare vancomycin hydrochloride-loaded carboxylated chitosan / chitosan quaternary ammonium salt nanoparticles (hereinafter referred to as VCM / CC-QAC-NPs) by ion cross-linking , namely the drug-loaded nanoparticles.

[0070] Be 6.8 mg by fixing the amount of VCM, the mass ratio of carboxylated chitosan and chitosan quaternary ammonium salt is respectively set to 10:1, 10:2, 10:3, 10:4, 10:5, obtains VCM / CC-QAC-NPs of different schemes form Examples 1-5.

[0071] The fo...

Embodiment 6

[0097] see Figure 5 , is a schematic flow chart of the preparation method of the hydrogel provided by the present invention. The preparation method of described hydrogel comprises the steps:

[0098] Step S1: Prepare a GP aqueous solution, and add 1 mL of the drug-loaded nanoparticles described in Example 4 into the aqueous sodium glycerophosphate solution, and mix evenly to obtain a solution C;

[0099] Specifically, GP is dissolved in 1 mL of ionized water, wherein GP can be α-GP or / and β-GP, preferably a mixture of α-GP and β-GP; in this embodiment, α-GP / β-GP ( w / w)=1:2-8,

[0100] Step S2: Dissolve an appropriate amount of CS and QAC in 0.1M acetic acid, and stir to obtain a clear solution D;

[0101] Specifically, CS / GP(w / w)=1.2-5:25; the solvent may be other organic carboxylic acids, sulfonic acids, sulfinic acids, etc. in addition to acetic acid.

[0102] Step S3: Add solution C dropwise to solution D in an ice bath, and stir for 15 minutes to obtain a drug-loaded ...

Embodiment 7-10

[0107] Using the hydrogel preparation method of Example 6, taking the amount of drug-loaded nanoparticles, CS / GP (w / w)=2:25 as quantitative, by changing the mass ratio of α-GP / β-GP, the example 7-10 VCM / CC-QAC-NPs / CS-QAC-Gel, and investigate its gel time at 37°C, as shown in Table 3. and please refer to Figure 7 , is a histogram of the gel time detection results of Examples 7-10 in the preparation method of the hydrogel provided by the present invention.

[0108] Table 3: Effect of α-GP / β-GP (w / w) on Gel Time

[0109] α-GP / β-GP (w / w) Gel time (min) Example 7 1:2 3.40 Example 8 1:4 3.6 Example 9 1:6 3.65 Example 10 1:8 4.3

[0110] combine Figure 7 It can be seen from Table 3 that the gelation time gradually increases with the decrease of the amount of α-GP, indicating that α-GP is beneficial to shorten the gelation time of the gel, and α-GP / β-GP=1:2(w / w) The minimum gelling time is 3.40min.

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Abstract

The invention discloses a drug-carried nanoparticle. The active ingredient of the drug-carried nanoparticle is vancomycin hydrochloride; a carrier material is formed by compounding carboxylation chitosan and chitosan quaternary ammonium salt according to a mass ratio of 10:(1 to 5) through ion crosslinking. The drug-carried nanoparticle has the advantages that the drug carrying rate on the vancomycin is obviously increased, the biodegradability is realized, and the drug-carried nanoparticle can be used for treating osteomyelitis. The invention also provides a preparation method and application of the drug-carried nanoparticle, and also provides a preparation method and application of aerogel based on the drug-carried nanoparticle. By utilizing the temperature-sensitive characteristic of the aerogel, the drug is administrated to the disease focal part, and is slowly released at the disease focal part, so as to improve the disease focal part.

Description

[0001] 【Technical field】 [0002] The invention relates to the field of biotechnology, in particular to a drug-loaded nanoparticle, a hydrogel and a preparation method and application thereof. [0003] 【Background technique】 [0004] Infected bone defect is the main clinical manifestation of traumatic osteomyelitis (Traumatic Osteomyelitis), which is prone to recurrent attacks, long-term non-healing, and high disability rate. Traffic accident injuries and high-energy injuries are increasing in modern society, leading to an increase in the incidence of severe and complicated traumatic osteomyelitis. Because it is usually combined with fractures, bone defects, and severe soft tissue damage, and the condition is repeated and changeable, the course of the disease is protracted and unhealed, scar hyperplasia around the lesion is obvious, and free bone fragments often form sequestrum, which makes it difficult for systemic antibiotic treatment alone to be effective. The residual rate...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/36A61K38/14A61P19/08A61P31/04A61L27/20A61L27/54A61L27/52A61L27/58
CPCA61K9/5161A61K38/14A61L27/20A61L27/52A61L27/54A61L27/58A61L2300/41A61L2300/412A61L2300/602A61L2300/624C08L5/08
Inventor 胡英杨云旭陶金
Owner ZHEJIANG PHARMA COLLEGE
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