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Tea tree flowering gene CsFT and encoding protein thereof

A technology that encodes proteins and genes, applied in the field of molecular biology

Active Publication Date: 2017-08-18
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no information about tea tree C Patent reports and publications related to gene sequences, protein sequences and their functions

Method used

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  • Tea tree flowering gene CsFT and encoding protein thereof
  • Tea tree flowering gene CsFT and encoding protein thereof
  • Tea tree flowering gene CsFT and encoding protein thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: tea tree C Gene cloning and sequence analysis

[0026] Longjing 43 was used as the test material to obtain tea tree C Different transcripts of genes obtained by transcriptome sequencing C Based on gene fragments, a 1050 bp gene was obtained using RACE technology C Full-length cDNA sequence information, C The full-length cDNA nucleotide sequence is shown in SEQ ID NO: 5. The prediction of its open reading frame (ORF) (http: / / www.ncbi.nlm.nih.gov / projects / gorf / ) showed that the gene might encode a small molecular weight protein containing 174 amino acids. Then cloned C The full-length ORF, ORF nucleotide sequence as shown in SEQ ID NO: 6, and pick a number of clones for sequencing verification, the results obtained two C A transcript of a gene with only 1 base difference between the two transcripts. But this 1 base difference leads to FT The 100th amino acid encoded by the gene is changed. One is phenylalanine (Phe) and the other is serine (Ser). ...

Embodiment 2

[0028] Embodiment 2: tea tree C Gene Expression Analysis

[0029] first with CsPTB1 As an internal reference gene, design universal primers for detection C Tissue-specific expression of genes and expression in leaves and axillary buds of different growth states. The results show, CThe gene was mainly highly expressed in leaves, flowers and buds, especially in buds, and its expression level was much higher than that in leaves. However, the expression in shoots was very low, while there was no expression in stems and roots ( Figure 4 ). this means C Genes are closely related to flower formation as well as bud development.

[0030] To further understand the two transcripts in tea tree CsFTa with cS We designed specific primers (Table 1) to distinguish the expression patterns of the two in different dormancy stages and under dormancy induction. The results showed that in axillary buds in paradormancy, endodormancy, ecodormancy and sprouting stages, CsFTa with ...

Embodiment 3

[0034] Example 3: Functional Verification and Phenotype Observation

[0035] At present, the transformation system of tea tree is still immature. As a model plant in woody plants, poplar has very comprehensive genetic information, and its dormancy mechanism has been studied most thoroughly. At the same time, both poplar and tea tree are woody plants with very close genetic background. Therefore, it is the most reliable to use the poplar transformation system to verify the function of the gene in the tea tree. The poplar clone 717 and clone 6 selected in this study are common transformation materials in the laboratory at present, with the advantages of easy phenotype identification, clear genetic background and high transformation rate. In addition, studies have found that dormancy (growth arrest and dormant bud formation) in poplar is only affected by photoperiod and has nothing to do with temperature (Resman et al. 2010). Therefore, the conditions of dormancy induction can ...

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Abstract

The invention relates to a tea tree flowering gene CsFT and an encoding protein thereof, belongs to the technical field of molecular biology, and provides transcripts CsFTa and CsFTb of the tea tree flowering gene CsFT and the encoding protein thereof. A plant expression carrier is established by the tea tree flowering gene, poplar trees with different genetic backgrounds are converted by agrobacterium, and an obtained transgenic poplar tree plant has obvious early flowering phenomenon, so that the gene has the function of inducing the early flowering of the poplar tree. The tea tree flowering gene has the advantages that after the plant expression carrier of the gene CsFT is applied to the tea tree, the flowering of the tea tree can be accelerated, the growth period of the tea tree is shortened, and the genetic improvement and variety breeding of the tea tree are promoted.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, in particular to tea tree flowering genes C and its encoded protein. Background technique [0002] In the last century, after long-term research on the mechanism of plant culture, it was found that the formation of plant flower buds is induced by a hormone-like substance produced in leaves. These substances are produced in mature leaves and then transported to terminal buds to perform flower bud induction functions. In recent years, the field of molecular biology and biochemistry has carried out in-depth research on this flowering-promoting substance, and found that this flowering-promoting substance is a Flowering locus T ( FT ) protein encoded by the gene. FT proteins have multiple functions in plant growth and development, not only as a transferable flowering induction signal, but also involved in the regulation of seasonal growth arrest and subsequent dormancy. Tea trees are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
CPCC07K14/415C12N15/8267C12N15/827
Inventor 郝心愿王新超杨亚军王璐
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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