Molecular marker for diagnosis and treatment of lung adenocarcinoma

A lung adenocarcinoma and substance technology, applied in the field of molecular markers for the diagnosis and treatment of lung adenocarcinoma

Active Publication Date: 2017-08-18
FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since lncRNA itself does not encode proteins, there are very few studies on its specific role in biological processes.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker for diagnosis and treatment of lung adenocarcinoma
  • Molecular marker for diagnosis and treatment of lung adenocarcinoma
  • Molecular marker for diagnosis and treatment of lung adenocarcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 Screening for Gene Markers Related to Lung Adenocarcinoma

[0076] 1. Sample collection

[0077] Paracancerous tissue and lung adenocarcinoma tissue samples were collected from 8 cases of lung adenocarcinoma. The tumor tissue specimens of lung adenocarcinoma were collected from the main tumor area, which was located at the junction of the middle and outer 1 / 3 of the tumor mass and normal tissue, and the obvious necrosis and calcification in the tumor center and normal lung tissue around the tumor were excluded; the normal lung tissue specimens adjacent to cancer were obtained from tumor There is no obvious change in the part above 5cm from the edge. All the above specimens were obtained with the consent of the organizational ethics committee.

[0078] 2. RNA sample preparation (using E.Z.N.A. miRNA kit for operation)

[0079] Introduce liquid nitrogen into the mortar, take the tissue obtained above and put it into the mortar, cut it into pieces in liquid ...

Embodiment 2

[0101] Example 2 QPCR sequencing verification of differential expression of LOC101926969 gene

[0102] 1. Large-sample QPCR verification of the differential expression of the LOC101926969 gene. According to the sample collection method in Example 1, 50 cases of lung adenocarcinoma paracancerous tissues and 50 cases of lung adenocarcinoma tissues were selected.

[0103] 2. The RNA extraction steps are as described in Example 1.

[0104] 3. Reverse transcription

[0105] 1) Reaction system:

[0106] Add 1 μl of RNA template, 1 μl of random primers, add double distilled water to 12 μl, mix well, centrifuge at low speed at 65°C for 5 min, then place on ice to cool.

[0107] Continue to add the following components to the 12 μl reaction solution:

[0108] 5× reaction buffer 4μl, RNase inhibitor (20U / μl) 1μl, 10mM dNTP mixture 2μl, AMV reverse transcriptase (200U / μl) 1μl; mix well and centrifuge;

[0109] 2) Reverse transcription reaction conditions

[0110] 5 minutes at 25°C,...

Embodiment 3

[0127] Example 3 Silencing of the LOC101926969 gene

[0128] 1. Cell culture

[0129] Human lung adenocarcinoma cell line A549 was incubated in RPMI1640 medium containing 10% fetal bovine serum and 1% P / S at 37°C, 5% CO 2 , Cultivated in an incubator with a relative humidity of 90%. Change the medium once every 2-3 days, and use 0.25% EDTA-containing trypsin for routine digestion and passage.

[0130] The cells in the culture flask were digested with trypsin and seeded in a 6-well plate to ensure that the number of cells was 2-8×10 5cells / well, add cell culture medium. Overnight, observe the cell density the next day, and transfection can be performed when the cell density is above 70%.

[0131] 2. Design of siRNA

[0132] Negative control siRNA sequence (siRNA-NC):

[0133] The sense strand is 5'-UUCUCCGAACGUGUCACGU-3' (SEQ ID NO.6)

[0134] The antisense strand is 5'-ACGUGACACGUUCGGAGAA-3' (SEQ ID NO.7)

[0135] siRNA-1:

[0136] The sense strand is 5'-UACAGAAAAUAAG...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a molecular marker for diagnosis and treatment of lung adenocarcinoma. The molecular marker used for diagnosis and treatment of lung adenocarcinoma has specificity and sensitivity. High expression of the molecular marker LOC101926969 in a patient suffering from the lung adenocarcinoma is discovered, expression of the gene is interfered by siRNA, cell proliferation can be inhibited, and the invasion number and transport number of cancer cells are reduced. On the basis, the invention provides a novel pathway for the diagnosis and treatment of the lung adenocarcinoma.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a molecular marker for diagnosis and treatment of lung adenocarcinoma, and the specific target is LOC101926969. Background technique [0002] Lung cancer is one of the most common malignancies in humans and the leading cause of cancer-related death. Although the diagnosis and treatment methods and technologies of lung cancer are continuously updated and improved, the overall prognosis is still unsatisfactory, and its mortality rate is still the highest among cancers, and the 5-year survival rate is less than 15%. Non-small cell lung cancer accounts for more than 85% of all lung cancer patients. In non-small cell lung cancer, lung adenocarcinoma is the most important part. The most effective treatment method is pneumonectomy plus appropriate adjuvant chemotherapy strategy , but even for stage I patients, about 25% of patients will relapse within a short period of time after the tumor is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68A61K48/00A61K45/00A61K31/713A61P35/00
CPCA61K31/713A61K45/00A61K48/005C12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 田子强李振华温士旺
Owner FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap