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A paper-based high-throughput photoelectrochemical biosensor construction method

A photoelectrochemical and biosensor technology, which is used in material analysis by observing the influence of chemical indicators, material analysis by optical means, and chemical reaction of materials, etc., can solve the problem of expensive instruments and complicated operations. , limit the wide application and development and other issues, to achieve the effect of saving detection costs, improving electrode conductivity, and improving detection sensitivity

Inactive Publication Date: 2020-11-10
UNIV OF JINAN
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods are expensive, complicated to operate, and even require professionals to operate, which is not conducive to the realization of portable high-throughput rapid on-site detection, which limits its wide application and development.

Method used

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  • A paper-based high-throughput photoelectrochemical biosensor construction method
  • A paper-based high-throughput photoelectrochemical biosensor construction method
  • A paper-based high-throughput photoelectrochemical biosensor construction method

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1: Simultaneous detection of MCF-7, K562, and HL-60 cancer cells in human blood.

[0037] (1) Design the hydrophobic wax batch printing pattern of the microfluidic paper chip on the computer, the style is as attached figure 1 As shown, the wax batch printing pattern consists of four parts, namely the working chip (A), the hollow channel chip (B), the semi-hydrophilic channel chip (C) and the auxiliary chip (D), 1 is the solution inlet, 2 4 is working electrode 1-3, 5 is hollow channel, 6-8 is semi-hydrophilic channel, 6 is printing 60% wax, 7 is printing 40% wax, 8 is printing 80% wax, and 9 is auxiliary electrode printing area; Then use a punch and laser cutting machine to attach figure 1 The middle grid area is punched and cut to prepare hollow channels; the chromatographic paper printed with hydrophobic wax batch printing patterns is placed in an oven and heated at 130 ℃ for 30 s to make the wax penetrate the inside of the paper fibers to form a completely hydrop...

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Abstract

The invention discloses a photoinduced electrochemical biological sensor with advantages of simple operation and high flux, which is successfully applied to simultaneously detect three types of cancer cells. The photoinduced electrochemical biological sensor is characterized in that a layer of gold nanoparticles is modified onto the surface of paper fiber through gold nanometer self-catalytic reduction; then, preparing cadmium sulfide-graphene-zinc oxide bar ternary composite materials at the surface of a paper working electrode which are modified by long gold, and using as photo-electrodes; using chemical light emitting to replace the traditional xenon lamp; by controlling the chemical light emitting reaction sequence, sequentially exciting photo-electrodes; sequentially detecting three photo-current peaks, so as to simultaneously detect the three types of cancer cells.

Description

Technical field [0001] The invention relates to the field of paper-based high-throughput photoelectrochemical analysis and detection, and more specifically to a method for constructing a paper microfluidic chip analysis and detection platform based on a chemiluminescence photoelectrochemical analysis method. Background technique [0002] Cancer is one of the most important diseases threatening human health today. The ability to make timely diagnosis in the early stage of cancer is one of the effective ways to improve the survival rate of patients. Cancer cells are a kind of mutated cells, which are the source of cancer. Different from normal cells, cancer cells have the three characteristics of infinite proliferation, transformation and easy metastasis. They can proliferate indefinitely and destroy normal cell tissues. Therefore, it is of great significance to construct a fast and accurate method for analyzing and detecting cancer cells. So far, various analysis methods have eme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/77G01N27/327G01N33/574G01N33/531
CPCG01N21/77G01N27/327G01N33/531G01N33/574G01N2021/7753
Inventor 王衍虎孔庆坤葛慎光于京华颜梅
Owner UNIV OF JINAN
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