Method for determining contents of psoralen and isopsoralen in fructus psoraleae through ASE (Accelerated Solvent Extraction)-HPLC (High Performance Liquid Chromatography)

A technology of isopsoralen and psoralen, which is applied in the field of chemical detection to achieve the effect of increasing viscosity, high sensitivity and reducing blocking

Inactive Publication Date: 2017-08-18
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, there are no bibliographical reports about the content determination of psoralen and isopsoralen in psoralen. In order to better control the

Method used

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  • Method for determining contents of psoralen and isopsoralen in fructus psoraleae through ASE (Accelerated Solvent Extraction)-HPLC (High Performance Liquid Chromatography)
  • Method for determining contents of psoralen and isopsoralen in fructus psoraleae through ASE (Accelerated Solvent Extraction)-HPLC (High Performance Liquid Chromatography)
  • Method for determining contents of psoralen and isopsoralen in fructus psoraleae through ASE (Accelerated Solvent Extraction)-HPLC (High Performance Liquid Chromatography)

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Embodiment 1

[0027] 1. Instruments and reagents

[0028] 1.1 Instruments:

[0029] Electronic analytical balance (XA205DU), ASE350 fast solvent extractor (DIONEX, USA), ThermoU3000UHPLC liquid chromatograph;

[0030] 1.2 Reagents (unless otherwise specified, all reagents used in this experiment are of analytical grade):

[0031] Water: Class I water in accordance with GB / T 6682;

[0032] Methanol (CH 4 O): chromatographically pure (for liquid chromatography);

[0033] Quartz sand: particle size 2mm.

[0034] 2 methods

[0035] 2.1 Preparation of reference solution:

[0036] Take an appropriate amount of psoralen and isopsoralen reference substances, add methanol to make a solution containing 20ug per 1ml, and obtain it.

[0037] 2.2 Preparation of the test solution

[0038] 2.2.1 "Chinese Pharmacopoeia" 2015 Edition Part I Preparation method for the test product:

[0039] Preparation of the test solution: Take about 0.5g of the powder of this product (passed through a No. 3 sieve)...

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Abstract

The invention discloses a method for determining the contents of psoralen and isopsoralen in fructus psoraleae through ASE (Accelerated Solvent Extraction)-HPLC (High Performance Liquid Chromatography), belongs to the field of chemical detection and aims to provide the method capable of simultaneously determining two effective ingredients of the psoralen and the isopsoralen in the fructus psoraleae. According to the method disclosed by the invention, an ASE method is adopted for extracting smashed fructus psoraleae by using methanol and preparing methanol extract liquor; an HPLC method is adopted for detecting the contents of the psoralen and the isopsoralen in the methanol extract liquor. The method disclosed by the invention can be used for determination of the contents of the effective ingredients in the fructus psoraleae.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to an ASE-HPLC method for detecting the content of psoralen and isopsoralen in psoralen. Background technique [0002] Psoralea (scientific name: Psoralea corylifolia Linn.), also known as: broken paper, po solid fat, mustard. It belongs to the order of Rosaceae, and the fruit is used as medicine. It has the effects of nourishing the liver and kidney, strengthening the spleen and replenishing qi, astringent essence and arresting nocturnal emission. Treat psoriasis and other skin diseases. [0003] In the prior art, there are no bibliographical reports about the content determination of psoralen and isopsoralen in psoralen. In order to better control the quality of this product and ensure that the medicine taken by the patient is really effective, it is necessary to modify the content determination method. research. Contents of the invention [0004] In view of the above-mentio...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 廖强韦日伟陈学松
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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