Gene recombination trypsin cell dissociation solution

Abstract

The invention discloses a gene recombination trypsin cell dissociation solution. The gene recombination trypsin cell dissociation solution comprises the following components according to a concentration unit: 50-70mg / L of dibasic potassium phosphate, 350-450mg / L of potassium chloride, 300-400mg / L of sodium hydrogen carbonate, 7000-9000mg / L of sodium chloride, 80-100mg / L of disodium hydrogen phosphate heptahydrate, 1000-2000mg / L of glucose, 200-500mg / L of EDTA-4Na, 1-60mg / L of gene recombination trypsin and the balance of water. The cell dissociation capability of the gene recombination trypsin cell dissociation solution is superior to that of the conventional trypsin; the gene recombination trypsin cell dissociation solution is capable of completely dissociating ordinary cells within three minutes; the cell dissociation process is relatively low in damage to the cells; the cells are inoculated after the cells are completely dissociated; the cells are high in living rate and good in growth conditions; the gene recombination trypsin cell dissociation solution does not need to be preserved at the temperature of 20 DEG C below zero; the using effect of the gene recombination trypsin cell dissociation solution is not changed after the gene recombination trypsin cell dissociation solution is preserved for 12 months at the temperature of 2-8 DEG C.

Description

technical field [0001] The invention relates to a gene recombined trypsin cell digestion liquid, which is used for digestion of adherent cells and belongs to the related fields of biopharmaceuticals, biotherapy and the like. Background technique [0002] Trypsin is a kind of animal-derived proteolytic enzyme, which is activated by trypsinogen secreted by animal pancreas. It can specifically hydrolyze the peptide bond at the terminal end of arginine and lysine. It is widely used in the field of biotechnology and medical field. [0003] At present, there are two main ways to obtain trypsin. The traditional way is to obtain trypsin from animals, by slaughtering cattle, sheep, pigs, etc., and extracting trypsin from their pancreas. In addition, the use of microbial fermentation to obtain trypsin is gradually favored by people because of its high efficiency and specificity. [0004] With the continuous development of biopharmaceuticals, trypsin has become the main raw material ...

AI Technical Summary

Problems solved by technology

Since trypsin is derived from living organisms and its components are complex, the donor is easily contaminated by pathogens. Once this pollution is not completely removed and is brought into the production of trypsin, it may lead to serious consequences.

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