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Anti-virus effect and application of novel anti-virus molecule Kdm6a (Lysine-specific deMethylase 6a)

A virus infection-related, type I technology, applied in the fields of biotechnology and medicine, can solve problems such as human health and national economic losses, and achieve the effect of resisting foreign virus infection and promoting production.

Inactive Publication Date: 2017-08-29
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Every kind of epidemic virus infection can cause great loss of human health and national economy. However, there is no specific drug against the virus so far. The focus of clinical treatment is on symptomatic and conservative treatment. Therefore, the molecular and immune mechanisms of viral diseases The study of medical mechanism and treatment strategy has great scientific significance and display significance

Method used

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  • Anti-virus effect and application of novel anti-virus molecule Kdm6a (Lysine-specific deMethylase 6a)
  • Anti-virus effect and application of novel anti-virus molecule Kdm6a (Lysine-specific deMethylase 6a)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Example 1: The promotion effect of Kdm6a on the production of macrophage IFN-β

[0110] Intraperitoneal injection of 2ml of 3% dehydrated thiolacetic acid medium, 4 days later, the mice were killed by cervical dislocation, soaked in 75% alcohol for 5-10 minutes, the abdomen of the mice was facing up, a small mouth was cut in the midline of the abdomen, and the skin was torn open. Fully expose the peritoneum. Then use a 20ml syringe to inject 10ml of serum-free 1640 medium. The needle tip is placed from the subperitoneum to the side of the peritoneal cavity, and the needle tip is facing upwards, avoiding the intestines and fat, and slowly sucking. At this time, the peritoneal lavage fluid contains a large number of macrophages. Put the collected peritoneal fluid into a 50ml centrifuge tube. Repeat this step once for a total of 20ml. Centrifuge the cell suspension at 800 g for 5 minutes, discard the supernatant, and resuspend with DMEM containing 10% FCS. The cell susp...

Embodiment 2

[0113] Example 2: The inhibitory effect of interfering with Kdm6a expression on the production of IFN-β in macrophages

[0114] The mouse macrophages obtained above were transfected with the interfering RNA (Si-Kdm6a) against Kdm6a (Si-Kdm6a) and the mock control (Si-mimetic) (the transfection reagent INTERFERin was purchased from Polyplus Company), and cultured in fresh RPMI 1640 after 6 hours base.

[0115] The interfering RNA (Si-Kdm6a) against Kdm6a and the mimic control (Si-mimetic) were purchased from Genephama Company, the sequence of Si-Kdm6a is shown in SEQ ID NO: 5 and SEQ ID NO: 6, and the Si-mimetic The sequences are shown in SEQ ID NO: 7 and SEQ ID NO: 8. During synthesis, 2 dTs were added at the 3' to make the sequences more stable.

[0116] The specific sequence is as follows:

[0117] Si-Kdm6a sequence:

[0118] 5'-GCUGCUACGAAUCUCUAAUTT-3' (Shunyi);

[0119] 5'-AUUAGAGAUUCGUAGCAGCTT-3' (antisense).

[0120] Si-mimetic sequence:

[0121] 5'-UUCUCCGAACGUGUC...

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Abstract

The invention relates to the fields of biotechnology and medicine, in particular to an anti-virus effect and application of a novel anti-virus molecule Kdm6a (Lysine-specific deMethylase 6a). The invention particularly relates to application of a Kdm6a protein or a Kdm6a coding sequence, a promoter thereof or an inhibitor thereof to a medicament or a kit for regulating and controlling generation of type I interferons, and further relates to application of the substances to the prevention and treatment of virus infective diseases, an application method and a corresponding medicament, pharmaceutical composition or kit. The novel anti-virus molecule Kdm6a can be used for regulating and controlling the generation of the type I interferons, resisting exogenous virus infection and treating relevant diseases caused by virus infection, can be alternatively applied to immunoregulation, and has a wide application prospect.

Description

technical field [0001] The present invention relates to the fields of biotechnology and medicine, in particular, the present invention relates to the use of lysine-specific demethylase 6a (Lysine-specific deMethylase 6a, Kdm6a) in resisting exogenous virus infection and treating related diseases caused by virus infection. Effects, mechanisms of action, methods of implementation and uses in disease. Background technique [0002] A virus is a tiny particle composed of nucleic acid (ribonucleic acid RNA or deoxyribonucleic acid DNA) as the core and protein as the shell (capsid). The nucleic acid can be divided into single strands or double strands, linear or spherical. Viruses are the smallest biological pathogens, ranging from 20nm to 300nm: the largest of which are poxviruses, such as vaccinia virus, which is 300nm × 200nm, barely visible under an ordinary light microscope; medium-sized ones such as influenza virus, with a diameter of 80 ~100nm; small viruses such as Japanes...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61K48/00A61K45/00A61K45/06A61P31/12
Inventor 李霞张迁李楠曹雪涛
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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