Production method of swimming crab roe oil with high EPA and DHA content
A production method and technology of Portunus crab, applied in the direction of fat oil/fat production, fat production, edible oil/fat, etc., can solve problems such as high water content, complicated process, low enzymatic hydrolysis degree, etc., to promote high-value utilization, The quality of the product is good, the color and permeability are good
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Embodiment 1
[0028] (1) Take crab roe: select fresh swimming crab, wash, remove the back shell, and absorb the crab roe;
[0029] (2) Drying: Spread the crab roe on a tray with a thickness of 0.8 cm, and dry it with air at a drying temperature of 45°C for 12 hours to obtain dried crab roe;
[0030] (3) Enzymolysis: crush the dried crab roe with water, mix the dried crab roe with water at a mass ratio of 1:5, and add a compound protease mixed with trypsin, neutral protease and papain, and the amount of trypsin added is 2000U / g dried crab roe, the amount of neutral protease added is 3000U / g dried crab roe, the amount of papain added is 3000U / g dried crab roe, after adjusting the pH value to 7, enzymolyze at 40°C for 3 hours, and then inactivate the enzyme to obtain the enzyme Solution;
[0031] (4) Centrifugal separation: centrifuge the enzymatic hydrolysis solution at a centrifugal speed of 4000r / min for 15 minutes, collect the upper liquid to obtain crude crab butter;
[0032] (5) Chole...
Embodiment 2
[0037] (1) Take crab roe: select fresh swimming crab, wash, remove the back shell, and absorb the crab roe;
[0038](2) Drying: spread the crab roe on a tray with a thickness of 1.2 cm, and dry it with air at a drying temperature of 50°C for 10 hours to obtain dried crab roe;
[0039] (3) Enzymatic hydrolysis: crush the dried crab roe with water, mix the dried crab roe with water at a mass ratio of 1:7, add a compound protease mixed with trypsin, neutral protease and papain, and the amount of trypsin added is 3000U / g dried crab roe, the amount of neutral protease added is 4000U / g dried crab roe, the amount of papain added is 4000 U / g dried crab roe, after adjusting the pH value to 8.5, enzymolyze at 45°C for 3.5h, and inactivate the enzyme after reaction Obtain enzymatic solution;
[0040] (4) Centrifugal separation: Centrifuge the enzymatic hydrolysis solution at a centrifugal speed of 4500r / min for 20 minutes, collect the upper liquid to obtain crude crab butter;
[0041]...
Embodiment 3
[0046] (1) Take crab roe: select fresh swimming crab, wash, remove the back shell, and absorb the crab roe;
[0047] (2) Drying: spread the crab roe on a tray with a thickness of 1 cm, and dry it with air at a drying temperature of 47°C for 11 hours to obtain dried crab roe;
[0048] (3) Enzymatic hydrolysis: crush the dried crab roe with water, mix the dried crab roe with water at a mass ratio of 1:6, add a compound protease mixed with trypsin, neutral protease and papain, and the amount of trypsin added is 2500U / g dried crab roe, the amount of neutral protease added is 3500 U / g dried crab roe, the amount of papain added is 3500 U / g dried crab roe, after adjusting the pH value to 8, enzymolyze at 42°C for 3.2 hours, and inactivate the enzyme after reaction Obtain enzymatic solution;
[0049] (4) Centrifugal separation: Centrifuge the enzymatic hydrolysis solution at a centrifugal speed of 4200r / min for 17min, collect the upper liquid to obtain crude crab butter;
[0050] (...
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