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Fibroin/carboxymethyl chitosan composite gel and preparation method thereof

A carboxymethyl chitosan and composite gel technology, which is applied in the field of silk fibroin/carboxymethyl chitosan composite gel and its preparation, can solve problems such as difficulty in large-scale application, and achieve short gel time , the effect of improving efficiency and high mechanical properties

Inactive Publication Date: 2017-09-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Carboxymethyl chitosan is a water-soluble chitosan derivative. Carboxymethyl chitosan composite materials have the advantages of guiding nerve regeneration, preventing tissue adhesion, promoting wound healing, and reducing scar formation. The resulting gel material is too strong in water absorption, brittle and brittle, making it difficult to apply on a large scale in the field of clinical medicine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation method of the silk fibroin / carboxymethyl chitosan composite gel in this embodiment includes the following steps in sequence.

[0046] (1) take by weighing the cocoon layer of 10g shredded and be 0.5% Na in massfraction 2 CO 3 Degumming twice in boiling aqueous solution, each time 30min, the silk fibroin fiber that obtains is wrung out after washing with deionized water, and dries in oven;

[0047] (2) Put the silk fiber obtained in step (1) in 9M LiBr solution, dissolve it at 60°C for 4 hours, and put the obtained liquid into a dialysis bag (molecular weight 8000-14000) in deionized water after filtration (3) An appropriate amount of carboxymethyl chitosan (90% degree of deacetylation, 90% degree of substitution) was dissolved in water to prepare a carboxymethyl chitosan solution with a concentration of 4% w / v.

[0048] (4) the concentration obtained in the step (2) is the carboxymethyl chitosan solution of 4% w / v in the silk fibroin solution of 0.5%w / v ...

Embodiment 2

[0055] The preparation method of the silk fibroin / carboxymethyl chitosan composite gel in this embodiment includes the following steps in sequence.

[0056] (1) take by weighing the cocoon layer of 10g shredded and be 0.5% Na in massfraction 2 CO 3 Degumming twice in boiling aqueous solution, each time 30min, the silk fibroin fiber that obtains is wrung out after washing with deionized water, and dries in oven;

[0057] (2) Put the silk fiber obtained in step (1) in 9M LiBr solution, dissolve it at 60°C for 4 hours, and put the obtained liquid into a dialysis bag (molecular weight 8000-14000) in deionized water after filtering After dialysis for 4 days, the water was changed continuously during the period, and the concentration of the finally obtained silk fibroin solution was 1.0% w / v.

[0058] (3) An appropriate amount of carboxymethyl chitosan (90% degree of deacetylation, 90% degree of substitution) was dissolved in water to prepare a carboxymethyl chitosan solution with...

Embodiment 3

[0065] The preparation method of the silk fibroin / carboxymethyl chitosan composite gel in this embodiment includes the following steps in sequence.

[0066] (1) take by weighing the cocoon layer of 10g shredded and be 0.5% Na in massfraction 2 CO 3 Degumming twice in boiling aqueous solution, each time 30min, the silk fibroin fiber that obtains is wrung out after washing with deionized water, and dries in oven;

[0067] (2) Put the silk fiber obtained in step (1) in 9M LiBr solution, dissolve it at 60°C for 4 hours, and put the obtained liquid into a dialysis bag (molecular weight 8000-14000) in deionized water after filtering After dialysis for 4 days, the water was changed continuously during the period, and the concentration of the finally obtained silk fibroin solution was 1.5% w / v.

[0068] (3) An appropriate amount of carboxymethyl chitosan (90% degree of deacetylation, 90% degree of substitution) was dissolved in water to prepare a carboxymethyl chitosan solution with...

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Abstract

The invention discloses fibroin / carboxymethyl chitosan composite gel and a preparation method of the composite gel. The composite gel mainly comprises fibroin and carboxymethyl chitosan at a mass ratio of (0.05-2):1. The method comprises the steps of performing silk layer treatment to obtain a fibroin fiber, preparing a fibroin solution, preparing a carboxymethyl chitosan solution, uniformly mixing the fibroin solution and the carboxymethyl chitosan solution to obtain a mixed solution, adding a certain amount of cross-linking agent, stirring the solution for a short time on a magnetic stirrer, immediately pouring the solution into a die for standing, and then immersing the solution into an alcohol aqueous solution to finally obtain the fibroin / carboxymethyl chitosan composite gel. According to the method, the gel with higher mechanical property and higher stability is prepared by a simpler technology and a shorter time; a novel clue is provided for improving a preparation method of fibroin composite gel; an application approach of the fibroin is further expanded.

Description

technical field [0001] The invention belongs to a processing technology of a regenerated silk fibroin solution, in particular to a silk fibroin / carboxymethyl chitosan composite gel and a preparation method thereof. Background technique [0002] Recently, gel materials have emerged in the field of biomedicine, and have been used in tissue engineering scaffolds, drug delivery carriers, and wound dressings, and have been favored by researchers. Many researchers have focused on the gel raw materials, which are cheap and easy to obtain. , natural polymer biomaterial with good biocompatibility and high safety performance. The regenerated silk fibroin solution is a thermodynamically unstable lysizing sol, which will transform from a random coil structure to a β-sheet structure and spontaneously transform into a gel state without any external action. Generally, this transformation takes 1 to 2 months, and the mechanical properties of the obtained gel are extremely poor, which great...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/09C08J3/24C08L5/08C08L89/00
CPCC08J3/095C08J3/24C08J2305/08C08J2389/00C08J2405/08C08J2489/00C08L5/08C08L89/00
Inventor 朱良均刘迪许宗溥胡豆豆杨明英
Owner ZHEJIANG UNIV
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