Method for efficiently extracting and purifying phellinus linteus polysaccharide through repeated compression and decompression

Abstract

The invention belongs to the technical field of extraction and purification of phellinus linteus polysaccharide, and provides a method for efficiently extracting and purifying the phellinus linteus polysaccharide through repeated compression and decompression. The method comprises the following steps: freezing, grinding and refining phellinus linteus fruit bodies, dispersing and swelling in deionized water, adding a surfactant to completely disperse the fine phellinus linteus to form an emulsion, and putting the emulsion into a reactor; on the premise that the stability of the emulsion is ensured, heating the emulsion to a certain temperature, and then repeatedly compressing and vacuumizing the emulsion to efficiently dissolve out the phellinus linteus polysaccharide; then destroying the emulsion by adding salt, centrifuging and collecting a supernatant to obtain the phellinus linteus polysaccharide. Compared with the conventional method, the method provided by the invention has the advantages as follows: the extracting and purifying efficiency is high, damage to the phellinus linteus polysaccharide is small, the activity of the phellinus linteus polysaccharide is kept better, and the purified phellinus linteus polysaccharide is applicable to a tumor treating medicine.

Description

technical field [0001] The invention relates to the technical field of purification of Phellinus polysaccharides, in particular to a method for efficiently purifying Phellinus polysaccharides through repeated compression and decompression. Background technique [0002] The remarkable efficacy of Phellinus Phellinus, a famous and precious traditional Chinese medicine, is mainly derived from the active polysaccharides in it. Phellinus polysaccharides are usually isolated from fruiting bodies, mycelia and fermentation broths, and are generally composed of monosaccharides with more than 10 molecules connected by glycosidic bonds. They can control cell division and differentiation, and regulate cell growth and aging. to the activity. Due to the hard texture and complex components of the fruiting bodies, it is difficult to extract Phellinus polysaccharides. The usual extraction methods are water extraction methods, including hot water extraction, enzyme extraction, microwave extr...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for repeatedly adding and reducing pressure to efficiently purify Phellinus polysaccharides, which can solve the relatively difficult defect of polysaccharide separation and purification in traditional methods. Most importantly, the creative application of repeated pressure and pressure methods for purification can ensure The purification process is carried out at a lower temperature to reduce the damage of Phellinus polysaccharide and retain its activity. At the same time, the dissolution efficiency of Phellinus polysaccharide is high and the yield is high