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Extraction method of gentiana urnula glycoside A, and applications of gentiana urnula glycoside A in preparation of cough-relieving drugs

An extraction method and technology of gentioside, applied in the field of extraction of gentioside A, can solve the problem of no chemical components of Gentiana daflora

Inactive Publication Date: 2017-09-15
上海中药标准化研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the chemical constituents of Gentiana grandis

Method used

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  • Extraction method of gentiana urnula glycoside A, and applications of gentiana urnula glycoside A in preparation of cough-relieving drugs
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  • Extraction method of gentiana urnula glycoside A, and applications of gentiana urnula glycoside A in preparation of cough-relieving drugs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Separation of urinogentioside A from Gentiana daflora medicinal material

[0014] Take 20g of the coarse powder of Gentiana gentiana, add 25 times the amount of water, heat and reflux extraction 3 times, 1 hour each time, take the supernatant after centrifugation, combine the supernatants, and absorb them all through the D101 macroporous resin column; Rinse the macroporous resin column with water, 35% ethanol aqueous solution, and 40% ethanol aqueous solution, collect the eluted fraction of 40% ethanol aqueous solution; concentrate and dry the 40% ethanol aqueous solution under reduced pressure to obtain 327.0 mg of extract, and use methanol for the extract After dissolving, use the methanol system to separate by gel Sephadex LH-20 column chromatography, collect the fractions of Gentiana gentiana A, concentrate and dry under reduced pressure to obtain 203.7 mg of extract, and then use ethyl acetate-methanol-water (10:1.5 : 1) The mobile phase of the system...

Embodiment 2

[0015] Embodiment 2: Separation of Unuginioside A from Udonia gentiana medicinal material

[0016] Take 20g of the coarse powder of Gentiana gentiana medicinal material, add 25 times the amount of water, heat and reflux for extraction 3 times, 1 hour each time, take the supernatant after centrifugation, and all the combined supernatants are absorbed by D101 macroporous resin column; , 35% ethanol aqueous solution, 40% ethanol aqueous solution to wash the macroporous resin column, collect 40% ethanol aqueous solution elution fraction; 40% ethanol elution fraction is concentrated and dried under reduced pressure to obtain 142.0 mg of extract, and the extract is dissolved in methanol Afterwards, the methanol system was used to separate by gel Sephadex LH-20 column chromatography, and the fractions of Gentiana Urnus A were collected, concentrated and dried under reduced pressure to obtain 81.6 mg of extract, and then ethyl acetate-methanol-water (10:1.5: 1) The mobile phase of the...

Embodiment 3

[0017] Embodiment 3: to the impact of mouse cough caused by concentrated ammonia water

[0018] (1) Experimental animals ICR mice, half male and half female, clean grade, 20±2g, provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine, license number: SCXK (Shanghai) 2013-0016.

[0019] (2) Reagent and instrument 25% concentrated ammonia water (analytical pure, Sinopharm Chemical Reagent Co., Ltd.); codeine phosphate tablets (30mg / tablet, China Pharmaceutical Industry Co., Ltd., batch number: 20130401); 402AI ultrasonic nebulizer (Jiangsu Yuyue Medical Equipment Co., Ltd.); the maximum daily dose of the positive drug codeine phosphate tablets is 8 tablets (30mg / tablet), and the equivalent conversion dose for mice is 30mg / kg; The dosage refers to the dosage of the active drug, and the high, middle and low dosages are 60mg / kg, 30mg / kg and 15mg / kg respectively.

[0020] (3) Experimental method The random block design method was adopted, a...

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Abstract

The invention relates to an extraction method of gentiana urnula glycoside A, and applications of gentiana urnula glycoside A in preparation of cough-relieving drugs. The extraction method comprises following steps: Gentiana szechenyii Kanitz or Gentiana urnula H. Smith crude powder is mixed with water, and is subjected to decoction or reflux extraction for 1 to 3 times, 1 to 3h each time, wherein the amount of water is 10 to 50 times of that of the Gentiana szechenyii Kanitz or Gentiana urnula H. Smith crude powder; an obtained mixture is subjected to centrifugation, obtained supernatant is mixed, and is subjected to macroporous resin column adsorption; macroporous resin column elution is carried out with water, 30 to 35% ethanol aqueous solution, and 40% ethanol aqueous solution successively, an elution fraction of the 40% ethanol aqueous solution is collected, and is subjected to condensation so as to obtain a 40% ethanol elution fraction; and the 40% ethanol elution fraction is subjected to gel Sephadex LH-20 column chromatography and silica-gel column chromatography separation, and obtained gentiana urnula glycoside A fractions are mixed so as to obtain a gentiana urnula glycoside A pure product. The gentiana urnula glycoside A can be used for treating cough as a cough-relieving drug, and can be prepared into pharmacologically acceptable dosage forms.

Description

technical field [0001] The present invention relates to a method for extracting urinogentioside A and its application for preparing cough medicine. Background technique [0002] For thousands of years, Tibetan medicine has played a huge role in relieving the suffering of Tibetan people. Gentiana szechenyii Kanitz and Gentiana urnula H.Smith are commonly used Tibetan medicines and belong to the genus Gentiana of the family Gentianae. They are mainly produced in Tibet and southwestern Qinghai, and they mostly grow in high mountains above 3,000 meters above sea level. Grassland or alpine gravel belt, alpine meadow and flowstone beach and other places. The white-flowered gentian, with Gentiana grandis as the mainstream species, has been used for respiratory diseases for more than a thousand years, and has a remarkable curative effect. "Standards of Tibetan Medicine" (1979 edited by the health bureaus of six provinces including Tibet) records that Gentiana gentiana (the prescri...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08A61K31/7048A61P11/14
CPCA61K31/7048C07H1/08C07H17/04
Inventor 吴立宏张莹莹王峥涛侴桂新杨少冉
Owner 上海中药标准化研究中心
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