Method for reconstitution of hair follicle in vivo

Abstract

Relating to the technical field of aesthetic and plastic surgery, the invention discloses a method for reconstitution of hair follicle in vivo. The method includes the steps of: firstly performing cell separation, culturing and amplifying freshly isolated cells, and using cell spheres or cell lumps for culture, conducting cell preparation before transplantation, directly taking freshly isolated skin single cells, or cultured and amplified epidermal and dermal cells mixed in proportion, or cell spheres or cell lumps, and resuspending them in a culture solution, narcotizing an immunodeficient mouse, using a syringe needle or cutisector to pierce a hole on the skin of the naked mouse, using a pipette gun head to transfer the well prepared cell suspension into the pierced hole, after transferring all the cells, performing covering with a silica gel membrane and conducting suturing, then laying a layer of vaseline gauze, conducting bandaging and timely observation and treatment, thus finding hair growth 3 weeks later. The method provided by the invention has the characteristics of simple operation, small trauma, high hair follicle formation efficiency, and large injectable cell number range, is suitable for injection of clump cells and a large number of cells, and is suitable for development and application in clinical practice or cosmetic medicine for treatment of hair loss diseases.

Description

technical field [0001] The invention relates to the technical field of cosmetic plastic surgery, in particular to a method for reconstructing hair follicles in vivo. Background technique [0002] At present, there are three commonly used methods for reconstructing hair follicles: small chamber method, injection method and transplantation method. These methods have their own defects and their practical value is not high. For example, the small chamber method is to implant a small chamber containing cells into the back of the mouse. This method takes a long time, and different sizes of small chambers need to be made according to different wound shapes. Before the wound heals, the tested animal needs to carry this heavy The small chamber is not suitable for clinical large-scale application. The injection method is to inject the mixed hair follicle cells into the mouse skin or subcutaneously. This method is simple and easy to operate, but it is not suitable for injecting agglom...

AI Technical Summary

Problems solved by technology

The injection method is to inject the mixed hair follicle cells into the mouse skin or subcutaneously. This method is simple and easy to operate, but it is not suitable for injecting agglomerated cells and a large number of cells, and the reconstructed hair growth direction is messy, and it is difficult to penetrate the skin and regenerate. Hair efficiency is low, therefore, this method cannot be used in clinical practice in the future

The transplantation method is a new transplantation method that has been improved recently. It can effectively regenerate hair, but it is similar to the small chamber method. It needs to remove a large area of ​​the host's skin (large trauma), and the transplantation operation is more complicated. It is practically applied, especially for the development of clinical treatment of hair loss. applications are limited