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A functional marker for identifying and screening tea trees with high catechin index and its application

A technology of catechins and tea trees, which is applied in biochemical equipment and methods, microbe determination/inspection, DNA/RNA fragments, etc. It can solve the problems of inability to accurately identify hybrid progeny individuals in the field and early identification

Active Publication Date: 2020-07-07
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, most of the identification of tea catechin content at home and abroad adopts biochemical determination method, which is affected by the development period of tea tree and living environment, and requires a certain amount of tea tree leaves, so it cannot accurately identify and hybridize wild resources. Early Identification of Generational Individuals

Method used

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  • A functional marker for identifying and screening tea trees with high catechin index and its application
  • A functional marker for identifying and screening tea trees with high catechin index and its application
  • A functional marker for identifying and screening tea trees with high catechin index and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Discovery of specific allelic differential sequences in the upstream regulatory region of F3'5'H, primer pairs and development of functional marker InDel-F3'5'H

[0020] 1. Test materials

[0021] Fuding Dabaicha and Xingyi No. 6 single plant 1 were selected as research materials.

[0022] 2. Determination of Catechin Content

[0023] Pick one bud and two leaves of the new shoots of tea trees in spring, dry them with hot air at 120°C for 5 minutes to fix the samples in time, and dry them at 75°C until they are fully dry and store them at low temperature. When it is to be determined, use mechanical grinding, pass through a 40-mesh sieve, and store it at low temperature for future use. Weigh 0.2g sample (accurate to 0.0001g), and place it in a 10mL centrifuge tube. Add 5 mL of 70% methanol solution preheated at 70°C, mix well, and then bathe in 70°C water bath. Water bath for 10 minutes, and stir 2-3 times in the middle. Centrifuge at 3,500r / min for 10min, ...

Embodiment 2

[0041] Example 2 Application of functional marker InDel-F3'5'H

[0042] 1. Test materials

[0043] The materials studied in this experiment are listed in Table 2, including 27 tea tree resources. The shoots with one bud and two leaves were collected in August for DNA extraction and identification of catechin traits.

[0044] Table 2 PCR amplification results and catechin index values ​​of 27 tea tree resources

[0045] Numbering resources name Catechin Index (CI) Value PCR amplification product size (bp) 1 Fuding white tea 0.432 152 2 Longjing 43 0.452 152 3 Yunkang No. 10 0.542 152 4 Zhongshan Thunder Tea 0.417 152 5 Changning 4 0.921 152 6 Xingyi No. 4 0.332 152 7 Mingtong group 0.247 152 8 Fuzao 2 0.249 152 9 Jinggu Laocang Group 0.668 152 10 Frost 0.196 152 11 Wanyuan 4 0.388 152 12 Hezhou tea 0.248 152 13 Hexian species 0.319 152 14 Chengbudong Tea...

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Abstract

The invention belongs to the biotechnological field, and relates to a functional marker for tea tree flavonoid 3'5'-hydroxylase genes. The upstream primer sequence of the functional marker is as shown by SEQ ID No.1 in the description, a downstream primer is as shown by SEQ ID No.2 in the description. The invention further provides application of the functional marker for the tea tree flavonoid 3'5'-hydroxylase genes in identifying and screening a tea tree with a high catechin index as well as an application method. The functional marker in the invention is used for molecular marker-assisted selection, and can quickly and accurately identify and screen out tea tree resources with the high catechin index (CI value is greater than 2.5).

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a functional marker for identifying and screening tea trees with high catechin index and its application and application method. Background technique [0002] Catechin is a derivative of 2-phenylbenzopyran. According to the number of hydroxyl groups in the B ring of catechin, the isomers at the 2 and 3 positions on the C ring, and whether the 3 position on the C ring is connected to gallic acid, etc. Divided into several components. According to the hydroxyl number of catechin B ring, catechin can be mainly divided into B ring dihydroxy catechin and trihydroxy catechin, among which epigallocatechin (EGC) and epigallocatechin gallate ( EGCG) belongs to the B-ring trihydroxycatechins, while epicatechin (EC) and epicatechin gallate (ECG) belong to the B-ring dihydroxycatechins. During the fermentation process of black tea, the formation of each theaflavin molecule requires 1 dihydroxyca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 金基强陈亮姚明哲马建强马春雷
Owner TEA RES INST CHINESE ACAD OF AGRI SCI