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Compound microbial agent of fermented organic fertilizer as well as preparation method and application of compound microbial agent
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A technology of compound microbial inoculants and strains, applied in the field of bio-organic fertilizers, can solve problems such as threats to human health and life safety, soil and water pollution, and uneven quality.
Inactive Publication Date: 2017-09-19
BEIJING DAWEIJIA BIOTECH SHARE CO LTD
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[0003] However, there are many types of organic fertilizers available in the market, and the quality is uneven. Some products are not fully fermented, and the pathogenic microorganisms or parasite eggs are not effectively killed. During the use, the soil and water sources are polluted, threatening human health and life. Safety
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experiment example 1
[0020] Experimental case 1. Production of microbial agents
[0021] The following strains are Bacillus subtilis WEI-2 CGMCC No.7744, Enterococcus faecium WEI-10 CGMCC No.7746, Lactobacillus plantarum WEI-70 CGMCC No.11747, Lactobacillus reuteri WEI-71 and Saccharomyces cerevisiae WJ-113, They were inoculated into their own slant medium, in which Saccharomyces cerevisiae (WJ-113) was cultured for 40 h, and other strains were cultured for 20 h. Then they were inoculated into the mixed fermentation medium from the slant, and cultured at 37°C and 180 rpm for 48 hours to prepare the microbial complex agent.
[0022] The mixed fermentation medium used in the above scheme is:
[0024] The characteristic of the above compound mic...
experiment example 2、1
[0027] Experimental case 2, preparation of 1# organic fertilizer
[0028] Take 50 kg of pig manure and 1 kg of sawdust and mix them evenly, then mix 10 kg of chopped straw, and at the same time add compound bacterial agent (5 L per ton of pig manure, no bacterial agent in the control group), adjust the content of 63% water and covered with plastic sheeting. During the composting process, measure the temperature regularly every day, measure the pH value once every two days, turn the compost twice on the 7th and 19th days, keep the water content between 55-65%, and the temperature drops below 50°C on the 29th day, Into the maturity stage.
experiment example 3、2
[0029] Experimental case 3, preparation of 2# organic fertilizer
[0030] Take 50 kg of pig manure and 1 kg of sawdust and mix them evenly, then mix 1 L of molasses, and at the same time add compound bacterial agent (5 L per ton of pig manure, no bacterial agent for the control group), adjust the water content to 65 % and covered with plastic sheeting. During the composting process, measure the temperature regularly every day, measure the pH value once every two days, turn the compost twice on the 11th and 16th days, keep the water content between 55-65%, and the temperature drops below 50°C on the 19th day, Into the maturity stage.
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Abstract
The invention provides a compound microbial agent of fermented organic fertilizer as well as a preparation method and application of the compound microbial agent. The compound microbial agent is prepared from bacillus subtilis, enterococcus faecium, lactobacillus plantarum, lactobacillus reuteri and saccharomyces cerevisiae. The preparation method comprises the following steps: simultaneously inoculating the five strains into a mixed fermentation culture medium, and fermenting for 48 hours to obtain fermentation broth with total viable bacterium amount of 3*10<8> to 4*10<8>CFU / mL; then inoculating the compound microbial agent into animal feces mixed with an auxiliary material according to the proportion of 1 to 2 percent, and subsequently fermenting for a certain time to obtain high-quality biological organic fertilizer. The compound microbial agent has the advantages of quick fermentation temperature rising and long high-temperature maintaining time; along with secondary temperature rising, quick composting of livestockmanure is promoted, and the content of humus is increased; soil fertility can be favorably improved, and the problem of environmental pollution can be solved; the compound microbial agent is suitable for innocent treatment and resource utilization of breeding manurepollution in livestock and poultry farms.
Description
Technical field: [0001] The invention relates to the technical field of bio-organic fertilizers, in particular to a microbial agent for fermenting bio-organic fertilizers Background technique: [0002] my country is a large livestock and poultry breeding country. The aquaculture industry has made outstanding contributions to the improvement of people's living standards. However, with the rapid expansion of the aquaculture industry, the discharge of livestock and poultry manure has increased sharply, causing serious pollution to the environment. In the past, the utilization of livestock and poultry manure was mainly to return to the field directly. With the development of biological fermentation technology, the use of microbial fermentation to produce organic fertilizer has attracted more and more attention. The use of livestock and poultry manure to produce organic fertilizer not only solves the problem of environmental pollution, but also provides high-quality fertilizer fo...
Claims
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