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Application of LOC101928926 gene

A gene and application technology, applied in lncRNALOC101928926 in the field of diagnosis and treatment of lung adenocarcinoma

Active Publication Date: 2017-09-22
FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are still few studies on the relationship between lncRNA and lung adenocarcinoma. Therefore, it is necessary to look for other key lncRNAs related to lung cancer, and understand their structural characteristics, biological characteristics, target genes and regulatory proteins, so as to study their role in lung cancer. The activation of oncogenes, the regulation of key signal transduction pathways, the growth and proliferation of tumor cells, and many other important aspects of the development of lung cancer provide a more reliable basis for gene diagnosis and targeted therapy of lung cancer

Method used

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  • Application of LOC101928926 gene
  • Application of LOC101928926 gene
  • Application of LOC101928926 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1 Screening for Gene Markers Related to Lung Adenocarcinoma

[0071] 1. Sample collection

[0072] Paracancerous tissue and lung adenocarcinoma tissue samples were collected from 8 cases of lung adenocarcinoma. The tumor tissue specimens of lung adenocarcinoma were collected from the main tumor area, which was located at the junction of the middle and outer 1 / 3 of the tumor mass and normal tissue, and the obvious necrosis and calcification in the tumor center and normal lung tissue around the tumor were excluded; the normal lung tissue specimens adjacent to cancer were obtained from tumor There is no obvious change in the part above 5cm from the edge. All the above specimens were obtained with the consent of the organizational ethics committee.

[0073] 2. RNA sample preparation (using E.Z.N.A. kit to operate)

[0074] Introduce liquid nitrogen into the mortar, take the tissue obtained above and put it into the mortar, cut it into pieces in liquid nitrogen...

Embodiment 2

[0096] Example 2 QPCR sequencing to verify the differential expression of the LOC101928926 gene

[0097] 1. Large-sample QPCR verification of the differential expression of the LOC101928926 gene. According to the sample collection method in Example 1, 50 cases of lung adenocarcinoma paracancerous tissues and 50 cases of lung adenocarcinoma tissues were selected.

[0098] 2. The RNA extraction steps are as described in Example 1.

[0099] 3. Reverse transcription

[0100] 1) Reaction system:

[0101] Add 1 μl of RNA template, 1 μl of random primers, add double distilled water to 12 μl, mix well, centrifuge at low speed at 65°C for 5 min, then place on ice to cool.

[0102] Continue to add the following components to the 12 μl reaction solution:

[0103] 5× reaction buffer 4μl, RNase inhibitor (20U / μl) 1μl, 10mM dNTP mixture 2μl, AMV reverse transcriptase (200U / μl) 1μl; mix well and centrifuge;

[0104] 2) Reverse transcription reaction conditions

[0105] 5 minutes at 25°...

Embodiment 3

[0122] Example 3 Overexpression of LOC101928926

[0123] 1. Cell culture

[0124] Human lung adenocarcinoma cell line A549 was incubated in RPMI1640 medium containing 10% fetal bovine serum and 1% P / S at 37°C, 5% CO 2 , Cultivated in an incubator with a relative humidity of 90%. Change the medium once every 2-3 days, and use 0.25% EDTA-containing trypsin for routine digestion and passage.

[0125] The cells in the culture flask were digested with trypsin and seeded in a 6-well plate to ensure that the number of cells was 2-8×10 5 cells / well, add cell culture medium. Overnight, observe the cell density the next day, and transfection can be performed when the cell density is above 70%.

[0126] 2. Construction of gene overexpression vector

[0127]Specific PCR amplification primers were synthesized according to the cDNA sequence of LOC101928926 (as shown in SEQ ID NO.1), and two restriction enzyme sites, HindIII and XhoI, were added to the 5' end primer and the 3' end prime...

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Abstract

The invention discloses application of an LOC101928926 gene which is used for preparing a product for diagnosing pulmonary adenocarcinoma. The invention further discloses application of the LOC101928926 gene in preparing or screening medicine for treating pulmonary adenocarcinoma. The LOC101928926 gene related to occurrence and development of pulmonary adenocarcinoma can serve as a biological marker of pulmonary adenocarcinoma to help a doctor make individualized treatment schemes, and meanwhile the pulmonary adenocarcinoma serves as a medicine target for treating tumors.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the use of the LOC101928926 gene, in particular to the use of lncRNA LOC101928926 in the diagnosis and treatment of lung adenocarcinoma. Background technique [0002] Lung cancer is the most common malignant tumor worldwide and an important cause of human death. According to reports, lung cancer accounts for 28% of male patients and 26% of female patients, ranking first among malignant tumors; and in terms of the incidence of malignant tumors, the incidence of lung cancer in both male and female patients is 14%. Both ranked second. Compared with other countries in the world, the incidence of lung cancer in the Chinese population is higher, and in recent decades, both male and female patients have shown an increasing trend year by year. Therefore, the molecular diagnosis and targeted therapy of lung cancer have always been the focus and hot issues of medical research all over the world. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K48/00A61P35/00
CPCA61K48/005C12Q1/6886C12Q2600/136C12Q2600/158
Inventor 田子强温士旺徐延昭
Owner FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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