A method for detecting 3-acetyl-2,5-dimethylthiophene
A technology based on dimethylthiophene and acetyl, which is applied in the field of analytical chemistry, can solve problems such as lack of research, and achieve the effects of high sensitivity, elimination of interference, and easy operation
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Embodiment 1
[0022] Example 1: Determination of 3-acetyl-2,5-dimethylthiophene in potato essence
[0023] (1) Sample preparation: take 1mL of potato essence, add deionized water to make the volume to 10mL, and obtain the sample solution;
[0024] (2) Sample determination: in the sample liquid of step (1), add 5mg magnetic Fe 3 o 4 Nanoparticles, vortexed for 1min, separated from solid and liquid by an external magnetic field, and took out the supernatant; Added a mixture of 500 μL octanoic acid and 100 μL TMN-6, vortexed for 1 min to form a cloudy solution, added 300 μL of ammonia water, vortexed for 1 min , to form uniform micelles, add 1.5mL of hydrochloric acid, vortex mix for 2min, centrifuge at 4000r / min for 8min, take out the mixture of caprylic acid and non-ionic surfactant TMN-6 in the upper layer, filter it through a 0.45μm organic phase filter membrane and analyze it by HPLC , The HPLC chromatographic conditions are: mobile phase: isocratic methanol-water solution, the ratio of...
Embodiment 2
[0025] Embodiment 2: Determination of 3-acetyl-2,5-dimethylthiophene in grape essence
[0026] (1) Sample preparation: take 2mL of grape essence, add deionized water to make the volume to 10mL, and obtain the sample solution;
[0027] (2) Sample determination: in the sample liquid of step (1), add 6mg magnetic Fe 3 o 4 Nanoparticles, vortexed for 2 minutes, separated from solid and liquid by an external magnetic field, and took out the supernatant; Added a mixture of 500 μL nonanoic acid and 100 μL TMN-6, vortexed and mixed for 2 minutes to form a cloudy solution, added 400 μL ammonia water, vortexed and mixed 1min to form uniform micelles, add 1mL of hydrochloric acid, vortex mix for 1min, centrifuge at 3000r / min for 10min, take out the mixture of nonanoic acid and non-ionic surfactant TMN-6 in the upper layer, filter through a 0.45μm organic phase filter membrane and use HPLC Analysis and chromatographic conditions were the same as in Example 1, and the content of 3-acetyl...
Embodiment 3
[0028] Example 3: Determination of 3-acetyl-2,5-dimethylthiophene in beef essence
[0029] (1) Sample preparation: Weigh 2g of beef essence, add 20g of acetone, ultrasonically extract for 30min, blow with nitrogen to 1mL, dilute to 10mL with deionized water, and set aside;
[0030] (2) sample determination: sample determination: in the sample liquid of step (1), add 8mg magnetic Fe 3 o 4 Nanoparticles, vortexed for 3 minutes, separated from solid and liquid by an external magnetic field, and took out the supernatant; Added a mixture of 500 μL heptanoic acid and 100 μL TMN-6, vortexed and mixed for 2 minutes to form a cloudy solution, added 500 μL ammonia water, vortexed and mixed 2min to form uniform micelles, add 2mL hydrochloric acid, vortex mix for 3min, centrifuge at 5000r / min for 5min, take out the mixture of heptanoic acid and non-ionic surfactant TMN-6 in the upper layer, filter through a 0.45μm organic phase filter membrane and use HPLC Analysis and chromatographic c...
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