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SNP marker combination and its application for traceability identification of beef cattle individual and meat products

An individual, beef technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as easy loss, difficulty in achieving traceability of meat products, and easy tampering of traceability information.

Active Publication Date: 2021-02-26
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the traditional electronic ear tag technology (radio frequency identification system) has problems such as easy loss, easy tampering of traceability information, and difficulty in traceability from meat products to livestock breeding conditions.

Method used

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  • SNP marker combination and its application for traceability identification of beef cattle individual and meat products
  • SNP marker combination and its application for traceability identification of beef cattle individual and meat products
  • SNP marker combination and its application for traceability identification of beef cattle individual and meat products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1. SNP marker combination for traceability identification of individual beef cattle and meat products

[0084]1. Sample collection and genomic DNA extraction

[0085] 1. Collection of samples

[0086] The samples of the present invention come from 192 individuals of 8 varieties in the Chinese market, namely Simmental cattle (n=43), Luxi cattle (n=34), Xinjiang black cattle (n=24), Limu Zan cattle (n=30), yaks (n=16), Nanyang yellow cattle (n=15), black Wagyu cattle (n=15), and Jiaxian red cattle (n=15). A large group consisting of 192 individuals of 8 breeds was a mixed group.

[0087] 2. Genomic DNA extraction

[0088] Blood samples were collected in EDTA anticoagulated blood collection tubes (5 mL), and blood DNA was extracted using GeneJET Genomic DNA purification Kit (Thermo Scientific, #k0721). Nanodrop2000c was used to measure DNA purity and concentration, and 0.8% agarose gel electrophoresis was used to measure the integrity of DNA fragments, and the ...

Embodiment 2

[0114] Example 2, the application of SNP site combination in the traceability identification of beef cattle products

[0115] 1. Sample collection and genomic DNA extraction

[0116] In a beef cattle breeding base (M), blood samples were randomly collected from 15 cattle including Simmental cattle, Luxi yellow cattle and Xinjiang black cattle by tail vein blood collection. The blood sample numbers were MB1-MB15, and recorded Ear tag number for each cow. When the above-mentioned cattle are slaughtered and marketed, the corresponding meat samples are taken, and the meat sample numbers are MM1-MM15. In addition, the meat samples of the other 5 cattle are randomly collected in the market, and the meat sample numbers are NM1-NM5. DNA extraction and quality control are the same as in Example 1.

[0117] 2. Genotype determination of samples and traceability identification of meat products

[0118] (1) Combination of 9 SNP sites

[0119] Using the following 9 SNP sites with the hi...

Embodiment 3

[0128] Example 3, the application of SNP locus combination in the identification of yak meat and other varieties of beef

[0129] 1. Sample collection and genomic DNA extraction

[0130] A total of 36 yak meat samples were collected from Qinghai, Gansu, Tibet, and Yunnan. A total of 50 samples of beef from other breeds including Simmental, Luxi cattle, Xinjiang black cattle, and Limousin cattle were collected for DNA analysis. Extraction and quality control, concrete operation is the same as embodiment 1.

[0131] 2. Genotype determination of samples and identification of yak meat from other varieties of beef

[0132] Select the sites in Table 3 that do not have polymorphisms in the yak population and have higher polymorphisms in other populations (other 30 SNP sites except rs29014752 and rs29020876 sites), and select among the 30 SNP sites The following 8 SNP loci are used to identify yak meat from other breeds of beef: rs2901990, rs29010374, rs29016185, rs29010006, rs29010...

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Abstract

The invention discloses a combination of SNP markers for the traceability identification of beef cattle individuals and meat products and its application. The SNP marker combination of the present invention contains 32 highly polymorphic sites distributed on 29 autosomes. The minimum allele frequencies of the combined loci were all greater than 0.35. Experiments have proved that this combination can be successfully applied to the individual identification and meat traceability of beef cattle other than yak breeds on the market, which is conducive to the realization of the whole chain traceability of breeding-slaughter-sales, and truly guarantees the safety of food.

Description

technical field [0001] The invention belongs to the field of individual identification and traceability detection, and in particular relates to a combination of SNP markers for traceability identification of beef cattle individuals and meat products and its application. Background technique [0002] Food traceability technology is considered to be the most reliable means to ensure food safety and protect the legitimate rights and interests of consumers. At present, the traditional electronic ear tag technology (radio frequency identification system) has problems such as easy loss, easy tampering of traceability information, and difficulty in traceability from meat products to livestock breeding. The gene traceability technology represented by SSR (Simple sequence repeat) and SNP (Single nucleotide polymorphism) has the advantages of uniqueness, inalterability, and hard-to-lose, and is gradually becoming a powerful supplement to the electronic ear tag traceability system. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/172
Inventor 杨曙明赵杰陈爱亮徐贞贞赵燕游新勇何雯菁赵璐瑶张莹
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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