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Probiotic compositions and methods of use

A technology of composition and bacteria, applied in the direction of drug combination, bacteria, application, etc.

Inactive Publication Date: 2017-09-26
WESTERN UNIV OF HEALTH SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Furthermore, the regulatory process for delivering probiotics as poultry feed additives or nutritional supplements takes years before a commercially viable product is approved

Method used

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  • Probiotic compositions and methods of use
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  • Probiotic compositions and methods of use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Growth of chicks vaccinated with the microbiota of adult chickens compared to non-vaccinated chicks. Several experiments were performed to verify the efficacy of inoculum prepared from chicken cecal material. The cecal material was administered to chicks that hatched that day.

[0050] To obtain the cecal material, the GI tract of adult birds was removed and the cecum was excised. The caecal portion was then homogenized into contents and scrapings, mixed 3:1 with phosphate-buffered saline (PBS), and 0.2 ml per bird was orally gavaged for inoculation of day-hatch chicks. Birds were weighed at weekly intervals and RFI values ​​were calculated on a per bird basis for the last two weeks of the experiment.

[0051] Approximately 80 newly hatched chicks were divided into two groups - those inoculated with cecal material from adult chickens, and a non-inoculated control group. The birds were then reared for 6 weeks on the ground typical of industry conditions. Chicks recei...

Embodiment 2

[0053] Identification and cultivation of gut probiotics. Samples were collected from the midgut and cecum of commercial chickens after 7, 21 and 41 days of incubation. The samples were then treated with chloroform to remove vegetative bacterial cells and incubated under anaerobic conditions. Briefly, feces were suspended in phosphate-buffered saline (PBS) and mixed with chloroform while shaking at room temperature for 60 min. The chloroform was separated by centrifugation at 2500 rpm for 20 min, and the treated sample was taken out from the culture solution with a pipette. Samples were grown on three different media and transferred until sterile colonies were obtained. Genomic DNA was sequenced on an Illumina MiSeq instrument with PE250 readout and data processing using standard genomics tools.

[0054] About 40 isolates have been obtained and characterized in several ways. First, the isolates were tested to see if they could inhibit the growth of various human and animal ...

Embodiment 3

[0058] A culture-based selective enrichment protocol for potential probiotics. A selective culture protocol was employed to selectively isolate Clostridium from the cecum of healthy chickens. Approximately 40 strains were placed in sterile culture as described in the previous examples. Briefly, exposure to formaldehyde, ethanol, and / or heat was used to kill vegetative cells, and spore-forming bacteria were selected for spreading on various growth media. Preliminary phylogenetic positions (based on 16S rRNA gene sequences) of the obtained isolates showed that most of the strains belonged to Clostridia. Of particular interest are some potentially novel taxa based on (<97% sequence similarity limit as a rule of thumb for species distinction) low 16S rRNA homology to the nearest known parent.

[0059] To assess their potential for competitive exclusion of pathogens, newly recovered isolates were tested against a panel of common enteric pathogens. Growth of at least one strain o...

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PUM

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Abstract

Compositions for oral administration to a young poultry bird, such as a chicken, that comprise cecal material from adult poultry birds and saline are disclosed. The compositions can be used to increase body weight gain in a poultry bird by administering them to a young bird by oral gavage. The compositions may also be used to increase feeding efficiency in a poultry bird. Further disclosed are compositions for application to poultry litter, for example, chicken litter, that are prepared using the cecal material from adult poultry bird. The compositions may be used to prevent or reduce colonization of the chicken gut by pathogenic bacteria. Also disclosed are method of selecting for bacterial strains with probiotic potential.

Description

technical field [0001] The present invention relates to probiotic compositions comprising caecal material from adult poultry and methods of use thereof. Background technique [0002] According to the National Agricultural Statistics Service, the cost per weight unit of poultry feed has more than doubled in the past decade and is expected to continue to increase in the future. Feed costs usually represent at least two-thirds of total production costs. Feed conversion efficiency (FCR) and growth rates have been greatly improved through optimization of feeding regimens, feed additives, and breeding, and important advances have been made in understanding the genetic factors underlying FCR. However, the room for further improvement of these factors is considered limited, and feed costs are likely to continue to increase from already high levels, with FCR being a key parameter for the success of the poultry industry. Water and land are the most important constraints on food supp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74
CPCA61K35/57A61P1/00A61P3/00A61K35/742A23K50/75A23K20/10A61K9/0053A61K35/741A61K2035/115C12N1/20
Inventor B·奥克利
Owner WESTERN UNIV OF HEALTH SCI
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