Sturgeon air bladder polypeptide health-care food capable of strengthening immune competence and preparation method thereof
A technology for enhancing immunity and health food, applied in food science and other directions, can solve the problems of low absorption and digestibility, high cost, large molecular weight, etc., and achieve the effects of no toxic side effects, simple operation, and enhanced immunity.
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Embodiment 1
[0018] A sturgeon swim bladder polypeptide health food for enhancing immunity, the ingredients and parts by weight are: 70-85 parts of sturgeon swim bladder polypeptide, 0.0002-0.0004 parts of isopropyl myristate, 10-20 parts of Ganoderma lucidum polysaccharide, and vitamin C0. 5~3 parts, polyphosphate 0.0001~0.0003 parts, soybean lecithin 1~10 parts. The combination of ingredients is scientific and reasonable, and it has a good promotion effect on cellular immunity, humoral immunity, macrophage phagocytosis and NK cell activity, and the effect of enhancing immunity of the composition is far better than that of a single ingredient; green and safe, non-toxic and side effects, Moreover, the immune enhancement effect is remarkable, and it has a good health care effect on the elderly, the infirm and postoperative patients. The isopropyl myristate and the polyphosphate can increase the digestion and absorption rate of the sturgeon swim bladder polypeptide health food by human cells...
Embodiment 2
[0026] 1) Take Sturgeon ( Acipenser schrenckii ) fish maw as a raw material, after homogenization, mixed with glycine-NaOH buffer solution with a pH of 10 according to the weight-to-volume ratio of 1g:25ml, homogenized, and then adjusted the pH value to 10 to obtain a mixed solution;
[0027] 2) Raise the temperature of the mixture to 50°C, stir and preheat for 10 minutes, and add alkaline protease (enzyme activity ≥ 1.9×10 4 U / g), the enzymolysis temperature is 50°C, and the enzymolysis time is 7h. The resulting enzymatic hydrolysis product was heated to 90°C, and kept at this temperature for 15 minutes, then cooled to room temperature, and then centrifuged to obtain the supernatant;
[0028] 3) The supernatant was passed through an ultrafiltration membrane with a molecular weight cut-off of 1 kDa, and the fraction less than 1 kDa was collected to make a solution with a concentration of 5 mg / ml, and the ultrafiltration enzymatic hydrolyzate was added to the Macroporous res...
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