Quantitative phase micro-imaging method based on annular programmable LED lighting
A technology of LED illumination and phase microscopy, applied in microscopes, instruments, optics, etc., can solve the problems of limiting the spatial resolution and measurement accuracy of the imaging system, unable to collect useful signals, and complexity, to improve compatibility and flexibility. The effect of improving the utilization rate of light energy
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[0020] like figure 2As shown, the present invention is a high-efficiency quantitative phase microscopy imaging system based on annular programmable LED illumination. The actual hardware platform of the imaging system includes an LED array, a stage, a condenser, a sample to be tested, a microscope objective lens, and an imaging tube. Mirror, camera, the system uses a ring-shaped illumination pattern and adds a condenser lens to the light path. The LED array is placed at the front focal plane of the condenser, and the center of the LED array is on the optical axis of the microscope objective, the back focal plane of the microscope objective coincides with the front focal plane of the imaging tube lens, and the imaging plane of the camera is placed in the imaging The position of the back focal plane of the tube lens; during imaging, the sample to be tested on the stage is adjusted to the position of the front focal plane of the microscope objective lens to form an infinity-corre...
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