Immobilized tannase as well as preparation method and application thereof

A tannase, tannase technology, applied in biochemical equipment and methods, immobilized on/in organic carriers, enzymes, etc., can solve the problem of immobilized tannase low tannin conversion rate, mono The problems of low conversion rate of nitric acid and high cost of use can achieve the effect of good carrier strength, not easy to break and lose, and less loss.

Active Publication Date: 2017-11-03
山东森久生物材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Adopt enzymatic method to also have two obvious problems: (1) adopt free tannase, use cost is higher; (2) adopt immobilized tannase, use cost is low, but tannin conversion rate is

Method used

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  • Immobilized tannase as well as preparation method and application thereof
  • Immobilized tannase as well as preparation method and application thereof
  • Immobilized tannase as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Preparation of amino carrier;

[0042] The dispersant and deionized water are used as the dispersed phase; the glycidyl methacrylate, divinylbenzene, porogen, and initiator are uniformly mixed to form the organic phase. The organic phase is added to the dispersed phase, the particle size is adjusted, and the suspension polymerization is carried out at a certain temperature. After the polymerization reaction is completed, rinse the porogen with acetone. Sieve out the appropriate particle size to obtain epoxy-based balls. The epoxy value of the epoxy base ball is 320umol / g; the pore size is 80nm; the turbidity is 180 during the strength test; the particle size is 400um; the water content is 55%. Reaction of epoxy-based spheres with ethylenediamine to obtain amine-based carrier.

[0043] (2) Pretreatment of amino-type carrier;

[0044] Put the amine-based carrier into the chromatography column, rinse from top to bottom with 2 times the volume of the carrier bed lay...

Embodiment 2

[0052] (1) Preparation of amino carrier;

[0053] Dispersant and deionized water are used as dispersed phase; ethylene glycol dimethacrylate, allyl trimeric isocyanate, divinylbenzene, porogen, and initiator are uniformly mixed to form an organic phase. The organic phase is added to the dispersed phase, the particle size is adjusted, and the suspension polymerization is carried out at a certain temperature. After the polymerization reaction is completed, rinse the porogen with acetone. Sieve out the appropriate particle size to obtain epoxy-based balls. The epoxy value of the epoxy-based ball is 330umol / g; the pore size is 70nm; the turbidity is 160 during the strength test; the particle size is 300um; the water content is 65%. Reaction of epoxy-based spheres with butanediamine to obtain amine-based carrier.

[0054] (2) Pretreatment of amino-type carrier;

[0055] Put the amine-based carrier into the chromatography column, rinse from top to bottom with 5 times the volume ...

Embodiment 3

[0063] (1) Preparation of amino carrier;

[0064] Use dispersant and deionized water as dispersed phase; mix glycidyl methacrylate, divinylbenzene, ethylene glycol dimethacrylate, allyl isocyanate, porogen, and initiator evenly, for the organic phase. The organic phase is added to the dispersed phase, the particle size is adjusted, and the suspension polymerization is carried out at a certain temperature. After the polymerization reaction is completed, rinse the porogen with acetone. Sieve out the appropriate particle size to obtain epoxy-based balls. The epoxy value of the epoxy-based ball is 330umol / g; the pore size is 70nm; the turbidity is 160 during the strength test; the particle size is 300um; the water content is 65%. The epoxy-type base ball is reacted with hexamethylenediamine to obtain the amine-type carrier.

[0065] (2) Pretreatment of amino-type carrier;

[0066] Put the amine-based carrier into the chromatography column, rinse from top to bottom with 6 time...

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Abstract

The invention discloses preparation and application technologies of immobilized tannase and in particular relates to preparation of durable immobilized tannase and a technological method for preparing gallic acid by hydrolyzing tannic acid through the immobilized tannase. When the immobilized tannase disclosed by the invention is used for producing the gallic acid, the problems of few catalysis batches, high tannic acid residues, low conversion rate and the like when previous tannase is applied can be solved; the invention provides the preparation of the durable immobilized tannase and a novel application technology of the durable immobilized tannase to production of the gallic acid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the preparation of a durable immobilized tannase and a new process for preparing gallic acid by using the immobilized tannase. Background technique [0002] China is a big gallica planting country, and the output of gallnut extract ranks among the top in the world. The main ingredient in gallnut extract is tannic acid. The tannic acid molecule is an ester structure of glucose and gallic acid. [0003] Gallic acid is an important chemical intermediate, usually prepared by hydrolysis of gallic acid. The processes for preparing gallic acid from tannic acid mainly include: (1) heating and hydrolysis under acidic or alkaline conditions; (2) biological fermentation; (3) enzymatic method. At present, the production process of several major domestic manufacturers still uses heating and hydrolysis under acid or alkaline conditions, but this process has high energy consumption, m...

Claims

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Application Information

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IPC IPC(8): C12N11/08C12P7/42
CPCC12N9/18C12N11/08C12P7/42C12Y301/0102
Inventor 林炳旺刘学
Owner 山东森久生物材料有限公司
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