Device for producing BC (bacterial cellulose) composite

A technology of bacterial cellulose and composite materials, which is applied in the methods of biological material sampling, biochemical fiber processing, and biochemical cleaning devices, etc., can solve the problem of insufficient mechanical strength of wet bacterial cellulose, inability to cover gaps in gauze, uneven distribution, etc. problem, to achieve the effect of promoting rapid reproduction and producing cellulose, reducing labor intensity, and increasing production area

Pending Publication Date: 2017-11-17
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In terms of medical dressings, due to the insufficient mechanical strength of wet bacterial cellulose, it is easy to break during use and cause inconvenience in use. To achieve the same mechanical properties as degreasing gauze, the cellulose film is required to reach a certain thickness, which will increase the hardness of the film. , the skin-adhesiveness decreased significantly
[0004] The existing methods of preparing bacterial cellulose membrane composite materials all use static culture technology, including placing the materials that need to be composited with bacterial cellulose in the culture solution for co-cultivation, and doping the bacterial cellulose membrane with organic polymers or inorganic substances by impregnation. Composite, the efficiency is not high; and because the cellulose is only formed on the surface of the medium, the cellulose composited by the static culture method is less, and the distribution is uneven, and it cannot cover most of the gaps in the materials that need to be composited, such as gauze. very long

Method used

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  • Device for producing BC (bacterial cellulose) composite
  • Device for producing BC (bacterial cellulose) composite
  • Device for producing BC (bacterial cellulose) composite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Such as Figure 1-3 As shown, the culture medium container 1 is an open rectangular parallelepiped container with a length of 200 meters, a width of 2 meters, and a height of 3 meters. The upper and lower rows of rolling shafts 11 in the container are parallel to each other with 1000, and the axis of the rolling shaft 11 is between the upper and lower The distance between the left and right rolling shafts 11 is 0.2 meters, and the roller diameter is 0.1 meters.

[0040] (1) Bacteria culture

[0041] Connect Acetobacter xylinum into 300mL liquid medium (per 1L of water, 400g glucose, 60g yeast extract, 100g tryptone, 1.15g citric acid, Na 2 HPO 4 2.7g, pH5.0, sterilized at 115°C for 30min) expansion culture, shaker culture at 30°C, 160r / min or static culture for 12h before use;

[0042] (2) Fermentation preparation of bacterial cellulose composite membrane

[0043] Using polyester fiber as the skeleton material 13, when using, the nutrient solution sterilized by high temperatur...

Embodiment 2

[0047] (1) Bacteria culture

[0048] The seed solution of Acetobacter xylinum was inserted into 300L of liquid culture medium (per 1L of water, 100g of maltose, 3g of peptone, and 5g of yeast extract, pH 4.5, and sterilization at 115°C for 30 min at 10% of the input amount; Or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, Na 2 HPO 4 2.7g, 1L water, pH5.0, sterilized at 115°C for 30min), the bioreactor is controlled at a constant temperature of 30°C;

[0049] (2) Fermentation preparation of bacterial cellulose composite membrane

[0050] Using calico as the skeleton material 13, transfer the liquid culture medium containing the production strain prepared in step (1) to figure 1 In the culture medium container 1 of the type, the frame material 13 is subjected to disturbance culture at a speed of 280m / h, and after dynamic culture at 30°C for 18 hours, the bacterial cellulose / cotton printed composite film can be obtained;

[0051] (3) Material handling

[0052] Remove t...

Embodiment 3

[0054] (1) Bacteria culture

[0055] The seed solution of Acetobacter xylinum was inserted into 300L of liquid culture medium (per 1L of water, 100g of maltose, 3g of peptone, and 5g of yeast extract, pH 4.5, and sterilization at 115°C for 30 min at 10% of the input amount; Or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, Na 2 HPO 4 2.7g, 1L water, pH5.0, sterilized at 115°C for 30min), the bioreactor is controlled at a constant temperature of 30°C;

[0056] (2) Fermentation preparation of bacterial cellulose composite membrane

[0057] Using medical gauze as the skeleton material 13, transfer the liquid medium containing the production strain prepared in step (1) to figure 1 In the type culture medium container 1, the skeleton material 13 is fermented at a vehicle speed of 104m / h for 48h to obtain a cellulose / gauze composite material;

[0058] (3) Material handling

[0059] Remove the prepared bacterial cellulose material from the collecting drum 16, rinse it with ...

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Abstract

The invention relates to a device for producing a BC (bacterial cellulose) composite. The device comprises a culture medium container, rolling shafts, a framework material, a feeding drum, a collecting drum, a guide roll and a press roll, wherein two rows of the rolling shafts are arranged in the culture medium container in parallel, the rolling shafts in each row are spaced in parallel, the rolling shafts in the two rows are staggered in parallel, and the framework material enters the culture medium container firstly through feeding by the feeding drum and guide by the guide roll, then is wound in a staggered manner along two rows of the rolling shafts and conveyed in the culture medium container in an S-shaped track through rolling by the rolling shafts and finally is pulled out of the culture medium container to penetrate through the press roll and be guided into the collecting drum to be wound and collected. Efficient, continuous and large-scale production of the BC composite is realized, the product composite quality is improved, bacterial breeding of a culture medium is accelerated, and the utilization efficiency of the culture medium is improved.

Description

Technical field [0001] The invention belongs to the technical field of bacterial cellulose production, and particularly relates to a device for producing bacterial cellulose composite materials. Background technique [0002] Bacterial Cellulose (BC for short) is a kind of pure cellulose produced by microorganisms, sometimes commonly known as "coconut fruit", which can be widely used in food, facial masks, high-strength functional materials, textiles, papermaking, artificial blood vessels, tissue engineering Stents, artificial skins and medical dressings. However, the existing shallow tray fermentation production equipment has low production efficiency and output, low mechanization degree, high labor intensity, and continuous production. Therefore, it is urgent to develop a new type of fermentation device with high output and high-efficiency continuous production. [0003] In terms of medical dressings, wet bacterial cellulose is inconvenient due to insufficient mechanical strengt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M15/05D06M16/00C12M1/00C12R1/02D06M101/06D06M101/10D06M101/32D06M101/34
CPCC12M23/18C12M33/18C12M41/24D06M15/05D06M16/00D06M2101/10D06M2101/06D06M2101/34D06M2101/32
Inventor 洪枫付勇陈琳
Owner DONGHUA UNIV
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