Processing method of fish soluble paste through biological enzymolysis
A processing method and biological enzyme technology, which are applied in the processing field of biological enzymatic hydrolysis of fish pulp, can solve the problems of insufficient enzymatic hydrolysis of protein powder, long production cycle and long enzymatic hydrolysis time, and achieve high enzymatic hydrolysis efficiency, short production cycle, Good quality product
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[0014] Example 1:
[0015] The invention relates to a processing method for biological enzymatic hydrolysis of fish lye, which comprises step-by-step enzymatic hydrolysis with pepsin and trypsin, and adding a protease activator while adding pepsin for enzymatic hydrolysis. Fish soluble slurry is diluted with water before adding protease for enzymatic hydrolysis, fish soluble slurry: water=1:20~40v / v. Pepsin and trypsin are more active in diluted fish lysate.
[0016] The amount of pepsin added is 0.5-1.9% of the volume of the fish-dissolved slurry before dilution, and the amount of protease activator added is 0.01-0.04% of the volume of the fish-dissolved slurry before dilution. The pepsin hydrolysis temperature is 35-40°C, the pH is 2.0-4.0, and the enzymolysis time is 6-9 minutes. After the end of pepsin enzymatic hydrolysis, use a boiling water bath to inactivate the enzyme for 4-8 minutes, and then add trypsin in an amount of 0.3-1.2% of the volume of the fish-dissolved ...
Example Embodiment
[0019] Example 2:
[0020] The invention relates to a processing method for biological enzymatic hydrolysis of fish lye, which comprises step-by-step enzymatic hydrolysis with pepsin and trypsin, and adding a protease activator while adding pepsin for enzymatic hydrolysis. Fish soluble slurry is diluted with water before adding protease for enzymatic hydrolysis, fish soluble slurry: water=1:30v / v. Pepsin and trypsin are more active in diluted fish lysate.
[0021] The amount of pepsin added was 1.2% of the volume of the fish-dissolved slurry before dilution, and the amount of protease activator added was 0.02% of the volume of the fish-dissolved slurry before dilution. The pepsin hydrolysis temperature was 37°C, the pH was 3.0, and the hydrolysis time was 8 minutes. After the end of pepsin enzymatic hydrolysis, the enzyme was inactivated in a boiling water bath for 5 minutes, and trypsin was added in an amount of 0.8% of the volume of the fish lysate before dilution. The tr...
Example Embodiment
[0024] Example 3:
[0025] The invention relates to a processing method for biological enzymatic hydrolysis of fish lye, which comprises step-by-step enzymatic hydrolysis with pepsin and trypsin, and adding a protease activator while adding pepsin for enzymatic hydrolysis. Fish soluble slurry is diluted with water before adding protease for enzymatic hydrolysis, fish soluble slurry: water=1:30v / v. Pepsin and trypsin are more active in diluted fish lysate.
[0026]The amount of pepsin added was 1.6% of the volume of the fish-dissolved slurry before dilution, and the amount of protease activator added was 0.03% of the volume of the fish-dissolved slurry before dilution. The pepsin hydrolysis temperature was 37°C, the pH was 3.0, and the hydrolysis time was 7 minutes. After pepsin enzymatic hydrolysis, use a boiling water bath to inactivate the enzyme for 5 minutes, and then add trypsin in an amount of 0.9% of the volume of the fish lysate before dilution. The trypsin hydrolys...
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