Enzyme-labeled antibody used for tilapia mossambica IgM detection and preparation method of enzyme-labeled antibody

Abstract

The invention relates to a method for preparing an enzyme-labeled antibody for tilapia IgM detection, which belongs to the field of biotechnology. Tilapia serum is collected, and protein G is used for affinity purification to obtain tilapia IgM antibody, and then the tilapia IgM New Zealand white rabbits were immunized with antibodies to obtain rabbit anti-tilapia IgG antibodies, and the rabbit anti-tilapia IgG antibodies were labeled with horseradish peroxidase to obtain enzyme-labeled secondary antibodies. Compared with the prior art, the present invention has beneficial effects: the present invention adopts the method of affinity chromatography to prepare high-concentration rabbit anti-tilapia IgM polyclonal antibody, and carries out horseradish peroxidase labeling and labeling after purification Determination of antibody concentration, the titer is greater than 1:128000, and it is used in the immune reaction through appropriate dilution to detect tilapia IgM, which is helpful for the large-scale prevention and control of tilapia streptococcosis.

Description

technical field [0001] The invention relates to an enzyme-labeled antibody for detecting tilapia IgM and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Tilapia (Tilapia), known as "white salmon", has the characteristics of high protein content, fast growth, strong disease resistance, and less environmental pollution. It is a high-quality aquaculture species recommended by the United Nations. Our country introduced Nile tilapia fingerling fish in 1978. In 1983, Xia Dequan's research group of Wuxi Freshwater Fishery Research Center of Chinese Academy of Fishery Sciences introduced Oria tilapia fingerling fish. Excellent strain. After more than 30 years of development, my country has become the country with the largest tilapia farming output, with an annual output of 1.5 million tons, accounting for 50% of the world's total output. The products are exported to the United States, the European Union, Russia and other countrie...

AI Technical Summary

Problems solved by technology

However, since 2009, tilapia streptococcus disease has continued to break out in the main tilapia breeding areas of my country every summer, and the cumulative mortality rate can reach 95%, with an annual loss of about 1 billion to 1.5 billion yuan, causing huge economic losses. Seriously endanger the development of tilapia aquaculture industry, so the prevention and control of tilapia streptococcosis is very necessary

[0003] The antibody type produced by jawed fish is mainly IgM. The preparation of high-purity tilapia IgM and its enzyme-labeled antibody is the basis for the study of tilapia immune response and immune detection methods, and provides technical support for the prevention and control of tilapia diseases , but there is no commercially available labeled secondary antibody against tilapia IgM on the market

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