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Method for increasing content of alpha-tocopherol in peanut

A technology of tocopherol and peanuts, applied in the field of increasing the content of α-tocopherol in peanuts, to achieve the effect of increasing the content of α-tocopherol, with low cost and simple method

Active Publication Date: 2017-11-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(Wu Fengfeng. The effect of germination on the main nutritional components, physiological functions and processing characteristics of brown rice [D]. Jiangnan University, 2013.) However, there are few articles and patents on the use of germination to increase α-tocopherol in peanuts

Method used

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  • Method for increasing content of alpha-tocopherol in peanut
  • Method for increasing content of alpha-tocopherol in peanut
  • Method for increasing content of alpha-tocopherol in peanut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Pre-treatment: select high-quality fresh peanut seeds of Yueyou No. 13 with full appearance, consistent size and no mildew, rinse with distilled water to remove surface impurities, drain and soak in sodium hypochlorite solution with a concentration of 1wt% for 10min for disinfection , rinse repeatedly with distilled water until the pH value is neutral, add 3 times the quality of peanuts in distilled water, and soak for 6 hours at 25°C in the dark;

[0033] (2) Germination treatment: place the peanuts treated in step (1) at 27°C and 95% humidity for 7 days in the dark, and spray distilled water every 1 hour, and each spray lasts 1 minute;

[0034] (3) Sample processing and determination: The peanut buds obtained in step (2) were vacuum freeze-dried to make the moisture content 2.74 wt%, pulverized at a high speed, passed through a 40-mesh sieve, and vacuum-packed and stored in a drying oven at 25°C. Accurately weigh 2g of peanut sprouts powder, add 10mL of n-hexane, vo...

Embodiment 2

[0036] (1) Pretreatment: select high-quality fresh peanut seeds of Yuhua No. 15 with full appearance, consistent size and no mildew, rinse with distilled water to remove surface impurities, drain and soak them in sodium hypochlorite solution with a concentration of 1.5 wt% for 5 minutes. Disinfection, rinse repeatedly with distilled water until the pH value is neutral, add 2 times the mass of peanuts in distilled water, and soak for 12 hours at 23°C in the dark;

[0037] (2) Germination treatment: place the peanuts treated in step (1) at 32°C and 100% humidity for 7 days in the dark, and spray distilled water every 2 hours for 2 minutes each time;

[0038] (3) Sample processing and determination: The peanut buds obtained in step (2) were vacuum freeze-dried to make the moisture content 2.52 wt%, pulverized at high speed, passed through a 40-mesh sieve, vacuum-packed and stored in a drying oven at 25°C. The method for determining the content of α-tocopherol in peanuts is the sa...

Embodiment 3

[0040] (1) Pre-treatment: Select high-quality fresh Luhua No. 11 peanut seeds with full appearance, consistent size and no mildew, rinse with distilled water to remove surface impurities, drain and soak in sodium hypochlorite solution with a concentration of 0.5 wt% for 15 minutes. Disinfection, rinse repeatedly with distilled water until the pH value is neutral, add 4 times the quality of peanuts in distilled water, and soak for 4 hours at 27°C in the dark;

[0041] (2) Germination treatment: place the peanuts treated in step (1) at 29°C and 90% humidity for 7 days in the dark, and spray distilled water every 3 hours for 2 minutes each time;

[0042] (3) Sample treatment and determination: The peanut buds obtained in step (2) were vacuum freeze-dried to make the moisture content 2.48 wt%, pulverized at high speed, passed through a 60-mesh sieve, vacuum-packed and stored in a drying oven at 25°C. The method for determining the content of α-tocopherol in peanuts is the same as ...

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Abstract

The invention discloses a method for increasing the content of alpha-tocopherol in peanut. The method comprises the following steps: washing peanut seeds with water, draining off the water and then soaking the peanut seeds in 0.5 to 1.5 weight percent sodium hypochlorite solution for 5 to 15 minutes for sterilizing; flushing with water until a pH value is neutral, and then adding water of which the mass is 2 to 4 times that of the peanut, and soaking the peanut seeds for 4 to 12 hours under the light-avoiding condition that the temperature is 20 to 30 DEG C; sprouting away from light for 7 days under the conditions that the temperature is 26 to 32 DEG C and the humidity is 70 to 100 percent, and spraying water once every 1 to 3 hours, wherein spraying is continuously performed for 1 to 2 minutes each time; carrying out vacuum freeze drying on peanut buds so as to enable the water content of the peanut buds to reach 2 to 3 weight percent, crushing, then passing through a sieve with 40 to 60 meshes, carrying out vacuum packaging, putting a packaged product into a drying oven, and then preserving for determination. The method disclosed by the invention has the advantages of simplicity, low cost, popularization, obvious effect and variety generality, and can be popularized and applied; in addition, the content of the alpha-tocopherol of the treated peanut is 178. 63mu g / g DW or above and is increased by at least 4.87 times.

Description

technical field [0001] The invention relates to the field of increasing the content of alpha-tocopherol in plants, in particular to a method for increasing the content of alpha-tocopherol in peanuts. Background technique [0002] Vitamin E, also known as tocopherol, is a naturally occurring fat-soluble vitamin, including two types of tocopherols and tocotrienols. According to the position and number of methyl groups, it is divided into 8 kinds of α-, β-, δ-, γ-tocopherol and α-, β-, δ-, γ-tocotrienol. Among them, α-tocopherol has the highest biological activity (Kimberly Kline, KarlaA. Law son, Wei ping Yu, et al. Vitamin E and Cancer[J]. Vitamin and Cancer, 2007, 76: 435-461.), priority Absorbed and utilized by human body (Zingg J M. Vitamin E: An over view of major research directions[J]. Molecular Aspects of Medicine, 2007, 28(5-6):400-422.). Peanut and soybean, rapeseed and sunflower are listed as the four major oil crops in the world. They are the largest oil crops an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L25/00
CPCA23L25/00A23V2002/00A23V2300/10
Inventor 刘国琴李淑莹
Owner SOUTH CHINA UNIV OF TECH